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Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis

Publication ,  Journal Article
Layfield, LJ; Kerns, BM; Conlon, DH; Iglehart, JD; Marks, JR; Dodge, RK
Published in: The Breast Journal
January 1, 1995

Abstract: Several clinicopathologic variables influence prognosis in breast cancer, including stage, histologic grade, nodal status, and tumor size. Multiple studies have shown an independent value of proliferation index as a prognostic variable for the stratification into favorable and unfavorable groups. The monoclonal antibody MIB‐1 reacts with the same antigen site, not epitope, as recognized by the Ki‐67 antibody. Like Ki‐67, MIB‐1 reacts with cells in the late G1, S, M and G2 phases of the cell cycle, but MIB‐1 has the advantage of reacting with formalin‐fixed, paraffin‐embedded material. The authors investigated the feasibility of using image analysis to quantitate the MIB‐1 antibody staining (proliferation index [PI]) and predict survival in a series of 230 patients with stage I and stage II breast cancer. In a univariate Cox regression model, larger values of MIB‐1 were related to shorter survival times (p < 0.001). Exploratory statistical procedures were used to categorize the patients into good, intermediate, and poor survival groups using the following proliferation indices as cut‐points: <5%, 5–11%, and >11 %, respectively. Higher clinical stage was associated with higher MIB‐1 values and shorter survival (p = 0.01, and p = 0.003, respectively). Tumor size (p = 0.02) and nodal status (p = 0.05) were also associated with higher values of MIB‐1. After adjusting for age, clinical stage, nodal status, and tumor size in a multivariate analysis, MIB‐1 retained its prognostic significance (p < 0.0001) when considered as either a continuous or categorical variable. There were no significant associations between MIB‐1 determined proliferation index and age (p = 0.54), histologic grade (p = 0.69), nuclear grade (p = 0.06) or the presence of vascular invasion (p =.66). There is a strong statistical relationship between cell proliferative activity, as determined by MIB‐1 expression, and survival in early stage breast cancer. Copyright © 1995, Wiley Blackwell. All rights reserved

Duke Scholars

Published In

The Breast Journal

DOI

EISSN

1524-4741

ISSN

1075-122X

Publication Date

January 1, 1995

Volume

1

Issue

6

Start / End Page

362 / 371

Related Subject Headings

  • Oncology & Carcinogenesis
  • 1701 Psychology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Layfield, L. J., Kerns, B. M., Conlon, D. H., Iglehart, J. D., Marks, J. R., & Dodge, R. K. (1995). Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis. The Breast Journal, 1(6), 362–371. https://doi.org/10.1111/j.1524-4741.1995.tb00263.x
Layfield, L. J., B. M. Kerns, D. H. Conlon, J. D. Iglehart, J. R. Marks, and R. K. Dodge. “Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis.” The Breast Journal 1, no. 6 (January 1, 1995): 362–71. https://doi.org/10.1111/j.1524-4741.1995.tb00263.x.
Layfield LJ, Kerns BM, Conlon DH, Iglehart JD, Marks JR, Dodge RK. Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis. The Breast Journal. 1995 Jan 1;1(6):362–71.
Layfield, L. J., et al. “Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis.” The Breast Journal, vol. 1, no. 6, Jan. 1995, pp. 362–71. Scopus, doi:10.1111/j.1524-4741.1995.tb00263.x.
Layfield LJ, Kerns BM, Conlon DH, Iglehart JD, Marks JR, Dodge RK. Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis. The Breast Journal. 1995 Jan 1;1(6):362–371.

Published In

The Breast Journal

DOI

EISSN

1524-4741

ISSN

1075-122X

Publication Date

January 1, 1995

Volume

1

Issue

6

Start / End Page

362 / 371

Related Subject Headings

  • Oncology & Carcinogenesis
  • 1701 Psychology