Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques
Publication
, Journal Article
Marszalek, PE; Oberhauser, AF; Carrion-Vazquez, M; Fernandez, JM
Published in: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
December 1, 1999
We use single molecule AFM techniques to trigger mechanical unfolding and mechanical unfolding intermediates in the immunoglobulin modules of an engineered polyprotein. Site directed mutagenesis of the immunoglobulin modules results in well defined mechanical phenotypes that are readily detectable with single molecule AFM techniques. In addition to examining the conformational changes of proteins, we can also use similar techniques to trigger chair to boat, and chair inversion transitions in the pyranose rings of single polysaccharide molecules. Our results demonstrate the use of an AFM to detect conformational changes in single molecules with a resolution similar to that of NMR or X-ray diffraction techniques.
Duke Scholars
Published In
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN
0589-1019
Publication Date
December 1, 1999
Volume
1
Start / End Page
78
Citation
APA
Chicago
ICMJE
MLA
NLM
Marszalek, P. E., Oberhauser, A. F., Carrion-Vazquez, M., & Fernandez, J. M. (1999). Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, 1, 78.
Marszalek, P. E., A. F. Oberhauser, M. Carrion-Vazquez, and J. M. Fernandez. “Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques.” Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings 1 (December 1, 1999): 78.
Marszalek PE, Oberhauser AF, Carrion-Vazquez M, Fernandez JM. Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;1:78.
Marszalek, P. E., et al. “Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques.” Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 1, Dec. 1999, p. 78.
Marszalek PE, Oberhauser AF, Carrion-Vazquez M, Fernandez JM. Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;1:78.
Published In
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN
0589-1019
Publication Date
December 1, 1999
Volume
1
Start / End Page
78