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Quantitative switch in integrin expression accompanies differentiation of F9 cells treated with retinoic acid.

Publication ,  Journal Article
Burdsal, CA; Lotz, MM; Miller, J; McClay, DR
Published in: Developmental dynamics : an official publication of the American Association of Anatomists
December 1994

F9 embryonal carcinoma cells resemble epithelial cells when in monolayer culture. After treatment with retinoic acid these cells differentiate into fibroblastic-like cells in a sequence that has been modeled as the mammalian equivalent of the differentiation from stem cells of the inner cell mass to parietal endoderm. This study examined the changes in integrin subtypes that accompany retinoic acid-induced differentiation of F9 cells. Although several integrins were found to be present on the surface of F9 cells and retinoic acid-induced (RA) cells, the two dominant integrins were alpha 3 beta 1 and alpha 5 beta 1. Differentiation of F9 cells resulted in about 10- to 25-fold increase in the amount of alpha 3 beta 1 integrin protein as measured by immunoprecipitation of cell surface labeled material. There was a corresponding several-fold reduction of alpha 5 beta 1 protein. The concentration of alpha 3 mRNA was about the same in F9 and RA cells while the concentration of alpha 5 mRNA dropped several-fold after retinoic acid treatment. Thus alpha 3 regulation appeared to be largely posttranscriptional while the drop in alpha 5 protein may have been a result of transcriptional down-regulation. Quantitative measurement of adhesion suggested that most of the F9 and RA cell-substrate adhesion to fibronectin or laminin is mediated by these integrins. They are the dominant integrins present, and antibodies to either these integrins or to the substrate blocked the adhesion. Despite the large switch in integrin subtype protein expression there was little difference between the two cell types in initial cell interactions when adhesive affinities were measured quantitatively. Also there was no difference between the two phenotypes in rate of initial adhesive strengthening. The phenotypic difference was first observed with later events in the attachment and spreading of the RA-treated cells to the substrate. These results show that retinoic acid treatment alters the amounts of alpha 5 and alpha 3 integrin subunits during the F9 to RA phenotypic switch. The data show that these integrins are important in the cell-substrate adhesion to fibronectin and laminin. They show, however, that the phenotypic changes observed with differentiation are not associated with the initial preferential adhesions to the substrate, but rather with consequences that alter the cytoskeletal architecture of the cell.

Duke Scholars

Published In

Developmental dynamics : an official publication of the American Association of Anatomists

DOI

EISSN

1097-0177

ISSN

1058-8388

Publication Date

December 1994

Volume

201

Issue

4

Start / End Page

344 / 353

Related Subject Headings

  • Tretinoin
  • Transcription, Genetic
  • Protein Processing, Post-Translational
  • Phenotype
  • Mice
  • Laminin
  • Integrins
  • Gene Expression
  • Fibronectins
  • Developmental Biology
 

Citation

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ICMJE
MLA
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Burdsal, C. A., Lotz, M. M., Miller, J., & McClay, D. R. (1994). Quantitative switch in integrin expression accompanies differentiation of F9 cells treated with retinoic acid. Developmental Dynamics : An Official Publication of the American Association of Anatomists, 201(4), 344–353. https://doi.org/10.1002/aja.1002010406
Burdsal, C. A., M. M. Lotz, J. Miller, and D. R. McClay. “Quantitative switch in integrin expression accompanies differentiation of F9 cells treated with retinoic acid.Developmental Dynamics : An Official Publication of the American Association of Anatomists 201, no. 4 (December 1994): 344–53. https://doi.org/10.1002/aja.1002010406.
Burdsal CA, Lotz MM, Miller J, McClay DR. Quantitative switch in integrin expression accompanies differentiation of F9 cells treated with retinoic acid. Developmental dynamics : an official publication of the American Association of Anatomists. 1994 Dec;201(4):344–53.
Burdsal, C. A., et al. “Quantitative switch in integrin expression accompanies differentiation of F9 cells treated with retinoic acid.Developmental Dynamics : An Official Publication of the American Association of Anatomists, vol. 201, no. 4, Dec. 1994, pp. 344–53. Epmc, doi:10.1002/aja.1002010406.
Burdsal CA, Lotz MM, Miller J, McClay DR. Quantitative switch in integrin expression accompanies differentiation of F9 cells treated with retinoic acid. Developmental dynamics : an official publication of the American Association of Anatomists. 1994 Dec;201(4):344–353.
Journal cover image

Published In

Developmental dynamics : an official publication of the American Association of Anatomists

DOI

EISSN

1097-0177

ISSN

1058-8388

Publication Date

December 1994

Volume

201

Issue

4

Start / End Page

344 / 353

Related Subject Headings

  • Tretinoin
  • Transcription, Genetic
  • Protein Processing, Post-Translational
  • Phenotype
  • Mice
  • Laminin
  • Integrins
  • Gene Expression
  • Fibronectins
  • Developmental Biology