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Distribution of amyloid precursor protein and amyloid-beta in ocular hypertensive C57BL/6 mouse eyes.

Publication ,  Journal Article
Kipfer-Kauer, A; McKinnon, SJ; Frueh, BE; Goldblum, D
Published in: Curr Eye Res
September 2010

PURPOSE: Amyloid precursor protein (APP) and amyloid-beta (Abeta) appear to participate in the pathophysiology of retinal ganglion cell (RGC) death in glaucoma. We, therefore, determined the distribution of APP and Abeta in the retinas of C57BL/6 mice after induction of chronic ocular hypertension. METHODS: Ocular hypertension was induced in one eye of three-month-old C57BL/6 mice by injection of hypertonic saline into episcleral veins. After 6 weeks of documented elevated intraocular pressure (IOP), retinas were fixed with 4% paraformaldehyde and processed for immunohistochemistry with antibodies including a polyclonal antibody to the C-terminus of Abeta 40 (Novartis 17-40/23) and a polyclonal antibody to the APP ectodomain (Novartis 474). Distribution and semiquantitative expression of APP and Abeta immunolabeling in ocular hypertensive and control retinas were graded in a masked fashion and compared. RESULTS: APP and Abeta immunoreactivity was found in the pia/dura, optic nerve (ON), and RGC layer of ocular hypertensive retinas, whereas APP and Abeta immunoreactivity in the contralateral control eyes was detected only in the pia/dura. Comparison of ocular hypertensive and control eyes for Abeta immunolabeling was significant in the ON and RGC layer (p < 0.05) whereas no significant difference was found when compared for APP staining. CONCLUSIONS: High Abeta and APP levels were seen in ocular hypertensive retinas, probably due to abnormal APP-splicing in the presence of elevated IOP.

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Published In

Curr Eye Res

DOI

EISSN

1460-2202

Publication Date

September 2010

Volume

35

Issue

9

Start / End Page

828 / 834

Location

England

Related Subject Headings

  • Tonometry, Ocular
  • Retinal Ganglion Cells
  • Retina
  • Pia Mater
  • Optic Nerve
  • Ophthalmology & Optometry
  • Ocular Hypertension
  • Mice, Inbred C57BL
  • Mice
  • Intraocular Pressure
 

Citation

APA
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MLA
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Kipfer-Kauer, A., McKinnon, S. J., Frueh, B. E., & Goldblum, D. (2010). Distribution of amyloid precursor protein and amyloid-beta in ocular hypertensive C57BL/6 mouse eyes. Curr Eye Res, 35(9), 828–834. https://doi.org/10.3109/02713683.2010.494240
Kipfer-Kauer, Anna, Stuart J. McKinnon, Beatrice E. Frueh, and David Goldblum. “Distribution of amyloid precursor protein and amyloid-beta in ocular hypertensive C57BL/6 mouse eyes.Curr Eye Res 35, no. 9 (September 2010): 828–34. https://doi.org/10.3109/02713683.2010.494240.
Kipfer-Kauer A, McKinnon SJ, Frueh BE, Goldblum D. Distribution of amyloid precursor protein and amyloid-beta in ocular hypertensive C57BL/6 mouse eyes. Curr Eye Res. 2010 Sep;35(9):828–34.
Kipfer-Kauer, Anna, et al. “Distribution of amyloid precursor protein and amyloid-beta in ocular hypertensive C57BL/6 mouse eyes.Curr Eye Res, vol. 35, no. 9, Sept. 2010, pp. 828–34. Pubmed, doi:10.3109/02713683.2010.494240.
Kipfer-Kauer A, McKinnon SJ, Frueh BE, Goldblum D. Distribution of amyloid precursor protein and amyloid-beta in ocular hypertensive C57BL/6 mouse eyes. Curr Eye Res. 2010 Sep;35(9):828–834.

Published In

Curr Eye Res

DOI

EISSN

1460-2202

Publication Date

September 2010

Volume

35

Issue

9

Start / End Page

828 / 834

Location

England

Related Subject Headings

  • Tonometry, Ocular
  • Retinal Ganglion Cells
  • Retina
  • Pia Mater
  • Optic Nerve
  • Ophthalmology & Optometry
  • Ocular Hypertension
  • Mice, Inbred C57BL
  • Mice
  • Intraocular Pressure