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QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans.

Publication ,  Journal Article
Evans, TG; McElrath, MJ; Matthews, T; Montefiori, D; Weinhold, K; Wolff, M; Keefer, MC; Kallas, EG; Corey, L; Gorse, GJ; Belshe, R; Graham, BS ...
Published in: Vaccine
February 28, 2001

Three separate studies were undertaken in HIV-1 uninfected persons to determine if the adjuvant QS-21 improves the magnitude or kinetics of immune responses induced by recombinant soluble gp120 HIV-1(MN) protein (rsgp120) immunization. The QS-21 was administered at two doses (50 and 100 microg), either alone or in combination with aluminum hydroxide (600 microg). At the highest doses of rsgp120 (100, 300, and 600 microg), QS-21 exerted no significant effect on either binding or neutralizing antibody titers. Antibody binding and neutralizing responses fell dramatically when rsgp120, formulated with alum alone, was given at low doses (3 and 30 microg). In contrast, antibody responses similar in titer to those in the high dose antigen groups were induced with the low dose rsgp120 formulated with QS-21. In addition, the lymphocyte proliferation and delayed type hypersensitivity skin testing were superior in the QS-21 recipients compared with the alum recipients at the low antigen doses. Moderate to severe pain was observed in majority of the volunteers receiving QS-21 formulations, and vasovagal episodes and hypertension were not infrequent. Thus, the use of QS-21 may provide a means to reduce the dose of a soluble protein immunogen.

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Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

February 28, 2001

Volume

19

Issue

15-16

Start / End Page

2080 / 2091

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • T-Lymphocytes, Cytotoxic
  • Saponins
  • Safety
  • Middle Aged
  • Lymphocyte Activation
  • In Vitro Techniques
  • Immunization
  • Hypersensitivity, Delayed
 

Citation

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Evans, T. G., McElrath, M. J., Matthews, T., Montefiori, D., Weinhold, K., Wolff, M., … NIAID AIDS Vaccine Evaluation Group, . (2001). QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans. Vaccine, 19(15–16), 2080–2091. https://doi.org/10.1016/s0264-410x(00)00415-1
Evans, T. G., M. J. McElrath, T. Matthews, D. Montefiori, K. Weinhold, M. Wolff, M. C. Keefer, et al. “QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans.Vaccine 19, no. 15–16 (February 28, 2001): 2080–91. https://doi.org/10.1016/s0264-410x(00)00415-1.
Evans TG, McElrath MJ, Matthews T, Montefiori D, Weinhold K, Wolff M, et al. QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans. Vaccine. 2001 Feb 28;19(15–16):2080–91.
Evans, T. G., et al. “QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans.Vaccine, vol. 19, no. 15–16, Feb. 2001, pp. 2080–91. Pubmed, doi:10.1016/s0264-410x(00)00415-1.
Evans TG, McElrath MJ, Matthews T, Montefiori D, Weinhold K, Wolff M, Keefer MC, Kallas EG, Corey L, Gorse GJ, Belshe R, Graham BS, Spearman PW, Schwartz D, Mulligan MJ, Goepfert P, Fast P, Berman P, Powell M, Francis D, NIAID AIDS Vaccine Evaluation Group. QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans. Vaccine. 2001 Feb 28;19(15–16):2080–2091.
Journal cover image

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

February 28, 2001

Volume

19

Issue

15-16

Start / End Page

2080 / 2091

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • T-Lymphocytes, Cytotoxic
  • Saponins
  • Safety
  • Middle Aged
  • Lymphocyte Activation
  • In Vitro Techniques
  • Immunization
  • Hypersensitivity, Delayed