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Cellular and humoral immune responses to a canarypox vaccine containing human immunodeficiency virus type 1 Env, Gag, and Pro in combination with rgp120.

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AIDS Vaccine Evaluation Group 022 Protocol Team,
Published in: J Infect Dis
February 15, 2001

Elicitation of both memory cytotoxic T cell responses and human immunodeficiency virus (HIV)-neutralizing antibodies are desirable characteristics of an HIV vaccine regimen. We studied a combination vaccine regimen consisting of a canarypox (CP) vector containing Env, Gag, and Pro, in combination with a recombinant gp120 subunit protein. Twenty-six of 42 subjects who received CP Vac-Env-Pro demonstrated in vitro CD8(+) T cell responses, versus 3 of 17 who received the control CP rabies or gp120 vaccine only (P=.0003); 15 of these 26 demonstrated a CD8(+) cytotoxic T lymphocyte (CTL) response on > or =2 occasions postvaccination. The frequency of CD8(+) CTL response to HIV antigens was similar between vaccinia-naive and vaccinia-immune persons. Rgp120 immunization did not increase the CD8(+) CTL response to HIV type 1 envelope proteins, but rgp120 boosting did markedly enhance the titer and frequency of neutralizing antibodies to the MN strain of HIV. Overall, the combination of a CP Gag, Pro, Env vector, in combination with recombinant gp120, resulted in neutralizing antibodies in 91% of subjects and CD8(+) T cell responses in 62% of subjects. A nonreplicating pox virus shuttle vector vaccine appears to be capable of eliciting CD8(+) CTL responses in most healthy volunteers, whether they were vaccinia naive or vaccinia immune.

Duke Scholars

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Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

February 15, 2001

Volume

183

Issue

4

Start / End Page

563 / 570

Location

United States

Related Subject Headings

  • Vaccines, Synthetic
  • Vaccination
  • T-Lymphocytes, Cytotoxic
  • Recombinant Proteins
  • Neutralization Tests
  • Microbiology
  • Immunization, Secondary
  • Humans
  • HIV-1
  • HIV Protease
 

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AIDS Vaccine Evaluation Group 022 Protocol Team, . (2001). Cellular and humoral immune responses to a canarypox vaccine containing human immunodeficiency virus type 1 Env, Gag, and Pro in combination with rgp120. J Infect Dis. United States. https://doi.org/10.1086/318523
AIDS Vaccine Evaluation Group 022 Protocol Team, Christine K. “Cellular and humoral immune responses to a canarypox vaccine containing human immunodeficiency virus type 1 Env, Gag, and Pro in combination with rgp120.J Infect Dis, February 15, 2001. https://doi.org/10.1086/318523.
AIDS Vaccine Evaluation Group 022 Protocol Team, Christine K. “Cellular and humoral immune responses to a canarypox vaccine containing human immunodeficiency virus type 1 Env, Gag, and Pro in combination with rgp120.J Infect Dis, vol. 183, no. 4, 15 Feb. 2001, pp. 563–70. Pubmed, doi:10.1086/318523.
Journal cover image

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

February 15, 2001

Volume

183

Issue

4

Start / End Page

563 / 570

Location

United States

Related Subject Headings

  • Vaccines, Synthetic
  • Vaccination
  • T-Lymphocytes, Cytotoxic
  • Recombinant Proteins
  • Neutralization Tests
  • Microbiology
  • Immunization, Secondary
  • Humans
  • HIV-1
  • HIV Protease