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Presynaptic delta opioid receptors regulate ethanol actions in central amygdala.

Publication ,  Journal Article
Kang-Park, M-H; Kieffer, BL; Roberts, AJ; Siggins, GR; Moore, SD
Published in: J Pharmacol Exp Ther
February 2007

Endogenous opioid systems are implicated in the reinforcing effects of ethanol consumption. For example, delta opioid receptor (DOR) knockout (KO) mice show greater ethanol consumption than wild-type (WT) mice (Roberts et al., 2001). To explore the neurobiological correlates underlying these behaviors, we examined effects of acute ethanol application in brain slices from DOR KO mice using whole-cell patch recording techniques. We examined the central nucleus of amygdala (CeA) because the CeA is implicated in alcohol reinforcement (Koob et al., 1998). We found that the acute ethanol effects on GABA(A) receptor-mediated inhibitory postsynaptic currents (IPSCs) were greater in DOR KO mice than in WT mice. Ethanol increased the frequency of miniature IPSCs (mIPSCs) significantly more in DOR KO mice than in WT mice. In CeA of WT mice, application of ICI 174864 [[allyl]2-Tyr-alpha-amino-isobutyric acid (Aib)-Aib-Phe-Leu-OH], a DOR inverse agonist, augmented ethanol actions on mIPSC frequency comparable with ethanol effects seen in DOR KO mice. Superfusion of the selective DOR agonist D-Pen(2),D-Pen(5)-enkephalin decreased the mean frequency of mIPSCs; this effect was reversed by the DOR antagonist naltrindole. These findings suggest that endogenous opioids may reduce ethanol actions on IPSCs of CeA neurons in WT mice through DOR-mediated inhibition of GABA release and that the increased ethanol effect on IPSCs in CeA of DOR KO mice could be, at least in part, due to absence of DOR-mediated inhibition of GABA release. This result supports the hypothesis that endogenous opioid peptides modulate the ethanol-induced augmentation of GABA(A) receptor-dependent circuitry in CeA (Roberto et al., 2003).

Duke Scholars

Published In

J Pharmacol Exp Ther

DOI

ISSN

0022-3565

Publication Date

February 2007

Volume

320

Issue

2

Start / End Page

917 / 925

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Receptors, Opioid, delta
  • Receptors, GABA-A
  • Pharmacology & Pharmacy
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Inhibitory Postsynaptic Potentials
  • Ethanol
  • Enkephalin, Leucine
 

Citation

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MLA
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Kang-Park, M.-H., Kieffer, B. L., Roberts, A. J., Siggins, G. R., & Moore, S. D. (2007). Presynaptic delta opioid receptors regulate ethanol actions in central amygdala. J Pharmacol Exp Ther, 320(2), 917–925. https://doi.org/10.1124/jpet.106.112722
Kang-Park, Maeng-Hee, Brigitte L. Kieffer, Amanda J. Roberts, George Robert Siggins, and Scott D. Moore. “Presynaptic delta opioid receptors regulate ethanol actions in central amygdala.J Pharmacol Exp Ther 320, no. 2 (February 2007): 917–25. https://doi.org/10.1124/jpet.106.112722.
Kang-Park M-H, Kieffer BL, Roberts AJ, Siggins GR, Moore SD. Presynaptic delta opioid receptors regulate ethanol actions in central amygdala. J Pharmacol Exp Ther. 2007 Feb;320(2):917–25.
Kang-Park, Maeng-Hee, et al. “Presynaptic delta opioid receptors regulate ethanol actions in central amygdala.J Pharmacol Exp Ther, vol. 320, no. 2, Feb. 2007, pp. 917–25. Pubmed, doi:10.1124/jpet.106.112722.
Kang-Park M-H, Kieffer BL, Roberts AJ, Siggins GR, Moore SD. Presynaptic delta opioid receptors regulate ethanol actions in central amygdala. J Pharmacol Exp Ther. 2007 Feb;320(2):917–925.

Published In

J Pharmacol Exp Ther

DOI

ISSN

0022-3565

Publication Date

February 2007

Volume

320

Issue

2

Start / End Page

917 / 925

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Receptors, Opioid, delta
  • Receptors, GABA-A
  • Pharmacology & Pharmacy
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Inhibitory Postsynaptic Potentials
  • Ethanol
  • Enkephalin, Leucine