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Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin.

Publication ,  Journal Article
Joëls, M; Madamba, SG; Moore, SD; Morrison, JH; Siggins, GR
Published in: Regul Pept
April 24, 1990

We have combined electrophysiology and immunohistochemistry to study the somatostatin (SS) innervation of neurons in the rat hippocampal slice. After recording the intracellular response of a pyramidal CA1 neuron in vitro to SS, Lucifer Yellow was injected into the cell and the slice fixed and processed for immunohistochemical localization of SS in the vicinity of the recorded neuron. Most pyramidal neurons (70%) responded to SS with a hyperpolarization associated with marked slowing of spontaneous discharge and reduced input resistance. SS-containing elements either crossed, ran parallel or seemingly terminated on the Lucifer Yellow-filled SS-responsive cell. These occurrences of close proximity of apparent pre- and postsynaptic elements were observed in all layers of the CA1 region and may represent synaptic terminations of SS elements on a pyramidal neuron that are likely to elicit membrane hyperpolarizations.

Duke Scholars

Published In

Regul Pept

DOI

ISSN

0167-0115

Publication Date

April 24, 1990

Volume

28

Issue

2

Start / End Page

215 / 221

Location

Netherlands

Related Subject Headings

  • Staining and Labeling
  • Somatostatin
  • Rats, Inbred Strains
  • Rats
  • Neurons
  • Membrane Potentials
  • Male
  • Isoquinolines
  • Immunohistochemistry
  • Hippocampus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Joëls, M., Madamba, S. G., Moore, S. D., Morrison, J. H., & Siggins, G. R. (1990). Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin. Regul Pept, 28(2), 215–221. https://doi.org/10.1016/0167-0115(90)90019-s
Joëls, M., S. G. Madamba, S. D. Moore, J. H. Morrison, and G. R. Siggins. “Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin.Regul Pept 28, no. 2 (April 24, 1990): 215–21. https://doi.org/10.1016/0167-0115(90)90019-s.
Joëls M, Madamba SG, Moore SD, Morrison JH, Siggins GR. Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin. Regul Pept. 1990 Apr 24;28(2):215–21.
Joëls, M., et al. “Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin.Regul Pept, vol. 28, no. 2, Apr. 1990, pp. 215–21. Pubmed, doi:10.1016/0167-0115(90)90019-s.
Joëls M, Madamba SG, Moore SD, Morrison JH, Siggins GR. Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin. Regul Pept. 1990 Apr 24;28(2):215–221.

Published In

Regul Pept

DOI

ISSN

0167-0115

Publication Date

April 24, 1990

Volume

28

Issue

2

Start / End Page

215 / 221

Location

Netherlands

Related Subject Headings

  • Staining and Labeling
  • Somatostatin
  • Rats, Inbred Strains
  • Rats
  • Neurons
  • Membrane Potentials
  • Male
  • Isoquinolines
  • Immunohistochemistry
  • Hippocampus