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In vitro inhibition of collagen cross links by catechol analogs.

Publication ,  Journal Article
Murray, JC; Lindberg, KA; Pinnell, SR
Published in: J Invest Dermatol
March 1977

Catechol analogs inhibit the formation of hydroxylysine-derived intermolecular collagen cross links in tissue cultures of chick embryo calvaria. Formation of intermolecular collagen cross links was measured following incorporation of [14C]lysine, reduction with sodium borohydride, and elution from an ion exchange column with a pyridine-formate gradient. Cultures grown in the presence of 10(-3) M catechol, 10(-3) M dopamine, 10(-3) M L-dopa, or 10(-3) M D,L-serine-(2,3,4-trihydroxybenzyl)-hydrazide demonstrated between 43 and 84% inhibition of hydroxylysine formation. Collagen biosynthesis was not diminished in these cultures as compared to controls without additions or with beta-aminopropionitrile when measured by collagenase digestion. The formation of hydroxylysine-derived intermolecular cross links was inhibited 34 to 93% for 5,5'-dihydroxylysinonorleucine and 7 to 71% for 5-hydroxylysinonorleucine. The catechol analogs also inhibit the activity of lysyl hydroxylase as measured by specific tritium release as triated water from an L-[4,5-3H]lysine-labeled unhydroxylated collagen substrate prepared from chick calvaria. Since catechol analogs inhibit the formation of hydroxylysine in a cell-free assay, these compounds must pass into the cells of calvaria in this culture system to inhibit intracellular hydroxylysine formation and subsequently to diminish the reducible intermolecular cross links of the newly synthesized collagen.

Duke Scholars

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

March 1977

Volume

68

Issue

3

Start / End Page

146 / 150

Location

United States

Related Subject Headings

  • Skull
  • Protein-Lysine 6-Oxidase
  • Norleucine
  • Levodopa
  • Hydroxylysine
  • Dopamine
  • Dipeptides
  • Dermatology & Venereal Diseases
  • Depression, Chemical
  • Culture Techniques
 

Citation

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Murray, J. C., Lindberg, K. A., & Pinnell, S. R. (1977). In vitro inhibition of collagen cross links by catechol analogs. J Invest Dermatol, 68(3), 146–150. https://doi.org/10.1111/1523-1747.ep12492467
Murray, J. C., K. A. Lindberg, and S. R. Pinnell. “In vitro inhibition of collagen cross links by catechol analogs.J Invest Dermatol 68, no. 3 (March 1977): 146–50. https://doi.org/10.1111/1523-1747.ep12492467.
Murray JC, Lindberg KA, Pinnell SR. In vitro inhibition of collagen cross links by catechol analogs. J Invest Dermatol. 1977 Mar;68(3):146–50.
Murray, J. C., et al. “In vitro inhibition of collagen cross links by catechol analogs.J Invest Dermatol, vol. 68, no. 3, Mar. 1977, pp. 146–50. Pubmed, doi:10.1111/1523-1747.ep12492467.
Murray JC, Lindberg KA, Pinnell SR. In vitro inhibition of collagen cross links by catechol analogs. J Invest Dermatol. 1977 Mar;68(3):146–150.
Journal cover image

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

March 1977

Volume

68

Issue

3

Start / End Page

146 / 150

Location

United States

Related Subject Headings

  • Skull
  • Protein-Lysine 6-Oxidase
  • Norleucine
  • Levodopa
  • Hydroxylysine
  • Dopamine
  • Dipeptides
  • Dermatology & Venereal Diseases
  • Depression, Chemical
  • Culture Techniques