Skip to main content
Journal cover image

Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet.

Publication ,  Journal Article
Sampey, BP; Vanhoose, AM; Winfield, HM; Freemerman, AJ; Muehlbauer, MJ; Fueger, PT; Newgard, CB; Makowski, L
Published in: Obesity (Silver Spring)
June 2011

Obesity has reached epidemic proportions worldwide and reports estimate that American children consume up to 25% of calories from snacks. Several animal models of obesity exist, but studies are lacking that compare high-fat diets (HFD) traditionally used in rodent models of diet-induced obesity (DIO) to diets consisting of food regularly consumed by humans, including high-salt, high-fat, low-fiber, energy dense foods such as cookies, chips, and processed meats. To investigate the obesogenic and inflammatory consequences of a cafeteria diet (CAF) compared to a lard-based 45% HFD in rodent models, male Wistar rats were fed HFD, CAF or chow control diets for 15 weeks. Body weight increased dramatically and remained significantly elevated in CAF-fed rats compared to all other diets. Glucose- and insulin-tolerance tests revealed that hyperinsulinemia, hyperglycemia, and glucose intolerance were exaggerated in the CAF-fed rats compared to controls and HFD-fed rats. It is well-established that macrophages infiltrate metabolic tissues at the onset of weight gain and directly contribute to inflammation, insulin resistance, and obesity. Although both high fat diets resulted in increased adiposity and hepatosteatosis, CAF-fed rats displayed remarkable inflammation in white fat, brown fat and liver compared to HFD and controls. In sum, the CAF provided a robust model of human metabolic syndrome compared to traditional lard-based HFD, creating a phenotype of exaggerated obesity with glucose intolerance and inflammation. This model provides a unique platform to study the biochemical, genomic and physiological mechanisms of obesity and obesity-related disease states that are pandemic in western civilization today.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Obesity (Silver Spring)

DOI

EISSN

1930-739X

Publication Date

June 2011

Volume

19

Issue

6

Start / End Page

1109 / 1117

Location

United States

Related Subject Headings

  • Weight Gain
  • Rats, Wistar
  • Rats
  • RNA, Messenger
  • Obesity
  • Metabolic Syndrome
  • Male
  • Macrophage Activation
  • Liver
  • Inflammation Mediators
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sampey, B. P., Vanhoose, A. M., Winfield, H. M., Freemerman, A. J., Muehlbauer, M. J., Fueger, P. T., … Makowski, L. (2011). Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet. Obesity (Silver Spring), 19(6), 1109–1117. https://doi.org/10.1038/oby.2011.18
Sampey, Brante P., Amanda M. Vanhoose, Helena M. Winfield, Alex J. Freemerman, Michael J. Muehlbauer, Patrick T. Fueger, Christopher B. Newgard, and Liza Makowski. “Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet.Obesity (Silver Spring) 19, no. 6 (June 2011): 1109–17. https://doi.org/10.1038/oby.2011.18.
Sampey BP, Vanhoose AM, Winfield HM, Freemerman AJ, Muehlbauer MJ, Fueger PT, et al. Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet. Obesity (Silver Spring). 2011 Jun;19(6):1109–17.
Sampey, Brante P., et al. “Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet.Obesity (Silver Spring), vol. 19, no. 6, June 2011, pp. 1109–17. Pubmed, doi:10.1038/oby.2011.18.
Sampey BP, Vanhoose AM, Winfield HM, Freemerman AJ, Muehlbauer MJ, Fueger PT, Newgard CB, Makowski L. Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet. Obesity (Silver Spring). 2011 Jun;19(6):1109–1117.
Journal cover image

Published In

Obesity (Silver Spring)

DOI

EISSN

1930-739X

Publication Date

June 2011

Volume

19

Issue

6

Start / End Page

1109 / 1117

Location

United States

Related Subject Headings

  • Weight Gain
  • Rats, Wistar
  • Rats
  • RNA, Messenger
  • Obesity
  • Metabolic Syndrome
  • Male
  • Macrophage Activation
  • Liver
  • Inflammation Mediators