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Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent

Publication ,  Journal Article
Christenson, RH; Clemmensen, P; Magnus Ohman, E; Toffaletti, J; Silverman, LM; Grande, P; Vollmer, RT; Wagner, GS
Published in: Clinical Chemistry
January 1, 1990

We compared relative increases in creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) after reperfusion in myocardial infarction for four popular methods: electrophoresis, immunoinhibition, the "Magic Lite" (Ciba-Corning) system, and the Stratus (Dade). In a method comparison study, we confirmed that all four methods correlated (r>0.95). Electrophoresis demonstrated the greatest scatter about the regression line, immunoinhibition the least. For CK-MB quantities near each method's "positive cutoff" indicating mycardial infarction, results by all methods agreed in 95% of samples. To characterize relative increases in CK-MB, we computer-fitted data obtained from each method for serial specimens collected from six acute myocardial infarction patients during myocardial reperfusion. Although for each individual patient the four methods appeared to exhibit parallelism, the methods differed significantly in terms describing their appearance rate, peak-time & falloff, and time-to-peak activity. Consistent with these data, we found that the relative CK-MB increases at various times after reperfusion, compared with baseline concentrations, are method-dependent. Therefore, when using CK-MB for indicating coronary patency, one must develop specific limits for each method utilized.

Duke Scholars

Published In

Clinical Chemistry

ISSN

0009-9147

Publication Date

January 1, 1990

Volume

36

Issue

8

Start / End Page

1444 / 1449

Related Subject Headings

  • General Clinical Medicine
  • 3205 Medical biochemistry and metabolomics
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
  • 1101 Medical Biochemistry and Metabolomics
  • 1004 Medical Biotechnology
 

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Christenson, R. H., Clemmensen, P., Magnus Ohman, E., Toffaletti, J., Silverman, L. M., Grande, P., … Wagner, G. S. (1990). Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent. Clinical Chemistry, 36(8), 1444–1449.
Christenson, R. H., P. Clemmensen, E. Magnus Ohman, J. Toffaletti, L. M. Silverman, P. Grande, R. T. Vollmer, and G. S. Wagner. “Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent.” Clinical Chemistry 36, no. 8 (January 1, 1990): 1444–49.
Christenson RH, Clemmensen P, Magnus Ohman E, Toffaletti J, Silverman LM, Grande P, et al. Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent. Clinical Chemistry. 1990 Jan 1;36(8):1444–9.
Christenson, R. H., et al. “Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent.” Clinical Chemistry, vol. 36, no. 8, Jan. 1990, pp. 1444–49.
Christenson RH, Clemmensen P, Magnus Ohman E, Toffaletti J, Silverman LM, Grande P, Vollmer RT, Wagner GS. Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent. Clinical Chemistry. 1990 Jan 1;36(8):1444–1449.

Published In

Clinical Chemistry

ISSN

0009-9147

Publication Date

January 1, 1990

Volume

36

Issue

8

Start / End Page

1444 / 1449

Related Subject Headings

  • General Clinical Medicine
  • 3205 Medical biochemistry and metabolomics
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
  • 1101 Medical Biochemistry and Metabolomics
  • 1004 Medical Biotechnology