Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

A Fas-associated death domain protein/caspase-8-signaling axis promotes S-phase entry and maintains S6 kinase activity in T cells responding to IL-2.

Publication ,  Journal Article
Arechiga, AF; Bell, BD; Leverrier, S; Weist, BM; Porter, M; Wu, Z; Kanno, Y; Ramos, SJ; Ong, ST; Siegel, R; Walsh, CM
Published in: J Immunol
October 15, 2007

Fas-associated death domain protein (FADD) constitutes an essential component of TNFR-induced apoptotic signaling. Paradoxically, FADD has also been shown to be crucial for lymphocyte development and activation. In this study, we report that FADD is necessary for long-term maintenance of S6 kinase (S6K) activity. S6 phosphorylation at serines 240 and 244 was only observed after long-term stimulation of wild-type cells, roughly corresponding to the time before S-phase entry, and was poorly induced in T cells expressing a dominantly interfering form of FADD (FADDdd), viral FLIP, or possessing a deficiency in caspase-8. Defects in S6K1 phosphorylation were also observed. However, defective S6K1 phosphorylation was not a consequence of a wholesale defect in mammalian target of rapamycin function, because 4E-BP1 phosphorylation following T cell activation was unaffected by FADDdd expression. Although cyclin D3 up-regulation and retinoblastoma hypophosphorylation occurred normally in FADDdd T cells, cyclin E expression and cyclin-dependent kinase 2 activation were markedly impaired in FADDdd T cells. These results demonstrate that a FADD/caspase-8-signaling axis promotes T cell cycle progression and sustained S6K activity.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

October 15, 2007

Volume

179

Issue

8

Start / End Page

5291 / 5300

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • Signal Transduction
  • S Phase
  • Ribosomal Protein S6 Kinases
  • Phosphorylation
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Interleukin-2
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Arechiga, A. F., Bell, B. D., Leverrier, S., Weist, B. M., Porter, M., Wu, Z., … Walsh, C. M. (2007). A Fas-associated death domain protein/caspase-8-signaling axis promotes S-phase entry and maintains S6 kinase activity in T cells responding to IL-2. J Immunol, 179(8), 5291–5300. https://doi.org/10.4049/jimmunol.179.8.5291
Arechiga, Adrian F., Bryan D. Bell, Sabrina Leverrier, Brian M. Weist, Melissa Porter, Zhengqi Wu, Yuka Kanno, et al. “A Fas-associated death domain protein/caspase-8-signaling axis promotes S-phase entry and maintains S6 kinase activity in T cells responding to IL-2.J Immunol 179, no. 8 (October 15, 2007): 5291–5300. https://doi.org/10.4049/jimmunol.179.8.5291.
Arechiga AF, Bell BD, Leverrier S, Weist BM, Porter M, Wu Z, et al. A Fas-associated death domain protein/caspase-8-signaling axis promotes S-phase entry and maintains S6 kinase activity in T cells responding to IL-2. J Immunol. 2007 Oct 15;179(8):5291–300.
Arechiga, Adrian F., et al. “A Fas-associated death domain protein/caspase-8-signaling axis promotes S-phase entry and maintains S6 kinase activity in T cells responding to IL-2.J Immunol, vol. 179, no. 8, Oct. 2007, pp. 5291–300. Pubmed, doi:10.4049/jimmunol.179.8.5291.
Arechiga AF, Bell BD, Leverrier S, Weist BM, Porter M, Wu Z, Kanno Y, Ramos SJ, Ong ST, Siegel R, Walsh CM. A Fas-associated death domain protein/caspase-8-signaling axis promotes S-phase entry and maintains S6 kinase activity in T cells responding to IL-2. J Immunol. 2007 Oct 15;179(8):5291–5300.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

October 15, 2007

Volume

179

Issue

8

Start / End Page

5291 / 5300

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • Signal Transduction
  • S Phase
  • Ribosomal Protein S6 Kinases
  • Phosphorylation
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Interleukin-2
  • Immunology