Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric Le(x)-antigen.

Publication ,  Journal Article
Matsushita, Y; Hoff, SD; Nudelman, ED; Otaka, M; Hakomori, S; Ota, DM; Cleary, KR; Irimura, T
Published in: Clin Exp Metastasis
1991

Immunochemical studies of human colorectal carcinoma with various monoclonal antibodies against Le(X)-related carbohydrate antigens previously revealed that the amount of sialyl-dimeric Le(X) antigen (NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-R: SLX) associated with metastatic lesions was greater than in the primary tumors. To assess whether an experimental model can be used to study the direct relationship between this carbohydrate antigen and the tumor cell's metastatic behavior, we selected variant cells with increased surface SLX from established human colon carcinoma cell line HT-29. The cells in the upper 5% or lower 5% population in fluorescence intensity after reacting with a monoclonal antibody, FH6, were retrieved separately by a fluorescence-activated cell sorter and propagated. After three- or four-times selection, we obtained stable cell lines with low and high cell surface SLX antigens (HT-29 M1 and HT-29 M2, respectively). Binding of monoclonal antibody FH6 was detected to glycolipids extracted from HT-29 M2 cells but not from HT-29 M1 cells. Glycoprotein components having reactivity with monoclonal antibody FH6 were below the detectable level. HT-29 M2 cells injected intrasplenically into nude mice showed a slightly reduced incidence of metastasis to lung, liver and lymph nodes than did HT-29 M1 cells. Subsequently we found that SLX antigen was not detectable by immunohistochemical examination of these tumor cells grown in nude mice. Re-established cell line from nude mice xenografts expressed SLX antigen in vitro.

Duke Scholars

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

1991

Volume

9

Issue

3

Start / End Page

283 / 299

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Molecular Sequence Data
  • Mice, Nude
  • Mice
  • Lewis X Antigen
  • Immunohistochemistry
  • Humans
  • Glycoproteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Matsushita, Y., Hoff, S. D., Nudelman, E. D., Otaka, M., Hakomori, S., Ota, D. M., … Irimura, T. (1991). Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric Le(x)-antigen. Clin Exp Metastasis, 9(3), 283–299. https://doi.org/10.1007/BF01753731
Matsushita, Y., S. D. Hoff, E. D. Nudelman, M. Otaka, S. Hakomori, D. M. Ota, K. R. Cleary, and T. Irimura. “Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric Le(x)-antigen.Clin Exp Metastasis 9, no. 3 (1991): 283–99. https://doi.org/10.1007/BF01753731.
Matsushita Y, Hoff SD, Nudelman ED, Otaka M, Hakomori S, Ota DM, et al. Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric Le(x)-antigen. Clin Exp Metastasis. 1991;9(3):283–99.
Matsushita, Y., et al. “Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric Le(x)-antigen.Clin Exp Metastasis, vol. 9, no. 3, 1991, pp. 283–99. Pubmed, doi:10.1007/BF01753731.
Matsushita Y, Hoff SD, Nudelman ED, Otaka M, Hakomori S, Ota DM, Cleary KR, Irimura T. Metastatic behavior and cell surface properties of HT-29 human colon carcinoma variant cells selected for their differential expression of sialyl-dimeric Le(x)-antigen. Clin Exp Metastasis. 1991;9(3):283–299.
Journal cover image

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

1991

Volume

9

Issue

3

Start / End Page

283 / 299

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Molecular Sequence Data
  • Mice, Nude
  • Mice
  • Lewis X Antigen
  • Immunohistochemistry
  • Humans
  • Glycoproteins