SUMO2/3 conjugation is an endogenous neuroprotective mechanism.
Publication
, Journal Article
Datwyler, AL; Lättig-Tünnemann, G; Yang, W; Paschen, W; Lee, SLL; Dirnagl, U; Endres, M; Harms, C
Published in: J Cereb Blood Flow Metab
November 2011
Small ubiquitin-like modifier (SUMO)2/3 but not SUMO1 conjugation is activated after transient cerebral ischemia. To investigate its function, we blocked neuronal SUMO2/3 translation through lentiviral microRNA delivery in primary cortical neurons. Viability was unaffected by SUMO2/3 silencing unless neurons were stressed by transient oxygen-glucose deprivation (OGD). Both 15 and 45 minutes of OGD were tolerated by control microRNA-expressing neurons but damaged >60% of neurons expressing SUMO2/3 microRNA. Damaging OGD (75 minutes) increased neuronal loss to 54% (control microRNA) and to 99% (SUMO2/3 microRNA). This suggests that activation of SUMO2/3 conjugation is an endogenous neuroprotective stress response.
Duke Scholars
Published In
J Cereb Blood Flow Metab
DOI
EISSN
1559-7016
Publication Date
November 2011
Volume
31
Issue
11
Start / End Page
2152 / 2159
Location
United States
Related Subject Headings
- Ubiquitins
- Sumoylation
- Small Ubiquitin-Related Modifier Proteins
- Oxygen
- Neurons
- Neurology & Neurosurgery
- MicroRNAs
- Mice, Inbred C57BL
- Mice
- Lentivirus
Citation
APA
Chicago
ICMJE
MLA
NLM
Datwyler, A. L., Lättig-Tünnemann, G., Yang, W., Paschen, W., Lee, S. L. L., Dirnagl, U., … Harms, C. (2011). SUMO2/3 conjugation is an endogenous neuroprotective mechanism. J Cereb Blood Flow Metab, 31(11), 2152–2159. https://doi.org/10.1038/jcbfm.2011.112
Datwyler, Anna Lena, Gisela Lättig-Tünnemann, Wei Yang, Wulf Paschen, Sabrina Lin Lin Lee, Ulrich Dirnagl, Matthias Endres, and Christoph Harms. “SUMO2/3 conjugation is an endogenous neuroprotective mechanism.” J Cereb Blood Flow Metab 31, no. 11 (November 2011): 2152–59. https://doi.org/10.1038/jcbfm.2011.112.
Datwyler AL, Lättig-Tünnemann G, Yang W, Paschen W, Lee SLL, Dirnagl U, et al. SUMO2/3 conjugation is an endogenous neuroprotective mechanism. J Cereb Blood Flow Metab. 2011 Nov;31(11):2152–9.
Datwyler, Anna Lena, et al. “SUMO2/3 conjugation is an endogenous neuroprotective mechanism.” J Cereb Blood Flow Metab, vol. 31, no. 11, Nov. 2011, pp. 2152–59. Pubmed, doi:10.1038/jcbfm.2011.112.
Datwyler AL, Lättig-Tünnemann G, Yang W, Paschen W, Lee SLL, Dirnagl U, Endres M, Harms C. SUMO2/3 conjugation is an endogenous neuroprotective mechanism. J Cereb Blood Flow Metab. 2011 Nov;31(11):2152–2159.
Published In
J Cereb Blood Flow Metab
DOI
EISSN
1559-7016
Publication Date
November 2011
Volume
31
Issue
11
Start / End Page
2152 / 2159
Location
United States
Related Subject Headings
- Ubiquitins
- Sumoylation
- Small Ubiquitin-Related Modifier Proteins
- Oxygen
- Neurons
- Neurology & Neurosurgery
- MicroRNAs
- Mice, Inbred C57BL
- Mice
- Lentivirus