Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Ribose-enhanced myocardial recovery following ischemia in the isolated working rat heart.

Publication ,  Journal Article
Pasque, MK; Spray, TL; Pellom, GL; Van Trigt, P; Peyton, RB; Currie, WD; Wechsler, AS
Published in: J Thorac Cardiovasc Surg
March 1982

Recovery for myocardial adenosine triphosphate (ATP) following moderate periods of ischemic is dependent upon the availability of adenosine monophosphate (AMP) and diphosphate (ADP) for rephosphorylation. Recovery of AMP and ADP levels following ischemia is, in turn, determined by the rates of salvage and de novo adenine nucleotide synthesis. Phosphoribosyl pyrophosphate (PRPP) availability is rate limiting in both salvage and de novo adenine nucleotide synthesis. Parenteral ribose infusions in rats have been documented to elevate myocardial PRPP levels with resultant enhancement of adenine nucleotide synthesis. In this study postischemic recovery of myocardial function and ATP levels in isolated, working rat hearts given ribose infusions before and after ischemia was compared with recovery in control hearts subjected to the same protocol without ribose administration. The mean percent of functional recovery in control hearts following 15 minutes of warm ischemia reached values of 56.7 +/- 4.1%, 63.5% +/- 4.3%, 65.9% +/- 4.6%, and 70.5% +/- 4.7% at 2, 5, 10, and 15 minutes of work following ischemia. Hearts perfused with ribose demonstrated improved mean percent return of function at similar intervals of postischemic work with values of 67.9% +/- 4.2%, 73.7% +/- 3.7%, 81.0% +/- 3.5% (* = p less than 0.02 versus control) *and 85.4% +/- 3.3%, *respectively. Determinations of myocardial ATP levels (mumoles/gm of dry weight) made at the end of 15 minutes of postischemic work were significantly higher (p less than 0.02) in the ribose-treated hearts (18.9 +/- 0.7) than in controls (16.3 +/- 0.6). Infusion of ribose before and after ischemia is a biochemically logical method of improving postischemic myocardial ATP and functional recovery by manipulation of adenine nucleotide synthetic pathways.

Duke Scholars

Published In

J Thorac Cardiovasc Surg

ISSN

0022-5223

Publication Date

March 1982

Volume

83

Issue

3

Start / End Page

390 / 398

Location

United States

Related Subject Headings

  • Ribose
  • Respiratory System
  • Rats, Inbred Strains
  • Rats
  • Phosphoribosyl Pyrophosphate
  • Perfusion
  • Myocardium
  • Male
  • Heart Arrest, Induced
  • Heart
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pasque, M. K., Spray, T. L., Pellom, G. L., Van Trigt, P., Peyton, R. B., Currie, W. D., & Wechsler, A. S. (1982). Ribose-enhanced myocardial recovery following ischemia in the isolated working rat heart. J Thorac Cardiovasc Surg, 83(3), 390–398.
Pasque, M. K., T. L. Spray, G. L. Pellom, P. Van Trigt, R. B. Peyton, W. D. Currie, and A. S. Wechsler. “Ribose-enhanced myocardial recovery following ischemia in the isolated working rat heart.J Thorac Cardiovasc Surg 83, no. 3 (March 1982): 390–98.
Pasque MK, Spray TL, Pellom GL, Van Trigt P, Peyton RB, Currie WD, et al. Ribose-enhanced myocardial recovery following ischemia in the isolated working rat heart. J Thorac Cardiovasc Surg. 1982 Mar;83(3):390–8.
Pasque, M. K., et al. “Ribose-enhanced myocardial recovery following ischemia in the isolated working rat heart.J Thorac Cardiovasc Surg, vol. 83, no. 3, Mar. 1982, pp. 390–98.
Pasque MK, Spray TL, Pellom GL, Van Trigt P, Peyton RB, Currie WD, Wechsler AS. Ribose-enhanced myocardial recovery following ischemia in the isolated working rat heart. J Thorac Cardiovasc Surg. 1982 Mar;83(3):390–398.
Journal cover image

Published In

J Thorac Cardiovasc Surg

ISSN

0022-5223

Publication Date

March 1982

Volume

83

Issue

3

Start / End Page

390 / 398

Location

United States

Related Subject Headings

  • Ribose
  • Respiratory System
  • Rats, Inbred Strains
  • Rats
  • Phosphoribosyl Pyrophosphate
  • Perfusion
  • Myocardium
  • Male
  • Heart Arrest, Induced
  • Heart