Scholars@Duke publication: Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae.

Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae.

Publication , Journal Article

Perfect, JR; Rude, TH; Penning, LM; Johnson, SA

Published in: Gene

December 1, 1992

We have cloned the phosphoribosyl anthranilate isomerase (PRAI)-encoding gene (TRP1) of Cryptococcus neoformans by genetic complementation in Saccharomyces cerevisiae. Sequence analysis of this gene revealed it to be 939 bp in length, and without known promoter or termination sequences. Unlike some of the filamentous fungi, where PRAI enzymatic activity is controlled by a trifunctional gene product, the C. neoformans PRAI appears to be unifunctional. PRAI of C. neoformans exhibits 39% amino acid (aa) sequence identity compared to the S. cerevisiae counterpart. The TRP1 gene of C. neoformans maps to different size chromosomes in strains with different serotypes. The cloning of this gene for vector constructions, and the demonstration that S. cerevisiae can be used as a surrogate for C. neoformans gene expression, should help with the molecular studies of this significant fungal pathogen in our increasing immunocompromised population.

Duke Scholars

Author John Robert Perfect Medicine, Infectious Diseases

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Published In

Gene

DOI

10.1016/0378-1119(92)90053-r

ISSN

0378-1119

Publication Date

December 1, 1992

Volume

122

Issue

Start / End Page

213 / 217

Location

Netherlands

Related Subject Headings

Sequence Homology, Amino Acid
Saccharomyces cerevisiae
Restriction Mapping
Molecular Sequence Data
Genetic Complementation Test
Genes, Fungal
Developmental Biology
DNA, Fungal
Cryptococcus neoformans
Cloning, Molecular

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Perfect, J. R., Rude, T. H., Penning, L. M., & Johnson, S. A. (1992). Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae. Gene, 122(1), 213–217. https://doi.org/10.1016/0378-1119(92)90053-r

Perfect, J. R., T. H. Rude, L. M. Penning, and S. A. Johnson. “Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae.” Gene 122, no. 1 (December 1, 1992): 213–17. https://doi.org/10.1016/0378-1119(92)90053-r.

Perfect JR, Rude TH, Penning LM, Johnson SA. Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae. Gene. 1992 Dec 1;122(1):213–7.

Perfect, J. R., et al. “Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae.” Gene, vol. 122, no. 1, Dec. 1992, pp. 213–17. Pubmed, doi:10.1016/0378-1119(92)90053-r.

Perfect JR, Rude TH, Penning LM, Johnson SA. Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae. Gene. 1992 Dec 1;122(1):213–217.