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Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation.

Publication ,  Journal Article
Napolitani, G; Bortoletto, N; Racioppi, L; Lanzavecchia, A; D'Oro, U
Published in: Eur J Immunol
October 2003

The role of src-family tyrosine kinases in LPS-induced DC maturation has not been fully addressed. We show that LPS induces activation of c-Src and Lyn in human DC. Inhibition of these kinasesby PP1 uncoupled LPS-induced cytokine production from the up-regulation of costimulatory molecules, resulting in DC still capable of stimulating T cell proliferation but much less efficient in inducing Th1 differentiation. This is the first example of a pharmacological inhibitor able to modulate the capacity of DC to induce a particular type of immune response. Inhibition of src-family kinases impaired phosphorylation and accumulation of c-Jun, leading to reduced formation of AP-1 complexes upon LPS stimulation. Thus, src-kinases control cytokine production in LPS-induced DC maturation through a timely formation of AP-1.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

October 2003

Volume

33

Issue

10

Start / End Page

2832 / 2841

Location

Germany

Related Subject Headings

  • src-Family Kinases
  • Up-Regulation
  • Transcription Factor AP-1
  • Th1 Cells
  • Repressor Proteins
  • Receptors, Chemokine
  • Receptors, CCR7
  • Proto-Oncogene Proteins c-jun
  • Phosphorylation
  • Phosphoproteins
 

Citation

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MLA
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Napolitani, G., Bortoletto, N., Racioppi, L., Lanzavecchia, A., & D’Oro, U. (2003). Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation. Eur J Immunol, 33(10), 2832–2841. https://doi.org/10.1002/eji.200324073
Napolitani, Giorgio, Nicola Bortoletto, Luigi Racioppi, Antonio Lanzavecchia, and Ugo D’Oro. “Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation.Eur J Immunol 33, no. 10 (October 2003): 2832–41. https://doi.org/10.1002/eji.200324073.
Napolitani G, Bortoletto N, Racioppi L, Lanzavecchia A, D’Oro U. Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation. Eur J Immunol. 2003 Oct;33(10):2832–41.
Napolitani, Giorgio, et al. “Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation.Eur J Immunol, vol. 33, no. 10, Oct. 2003, pp. 2832–41. Pubmed, doi:10.1002/eji.200324073.
Napolitani G, Bortoletto N, Racioppi L, Lanzavecchia A, D’Oro U. Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation. Eur J Immunol. 2003 Oct;33(10):2832–2841.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

October 2003

Volume

33

Issue

10

Start / End Page

2832 / 2841

Location

Germany

Related Subject Headings

  • src-Family Kinases
  • Up-Regulation
  • Transcription Factor AP-1
  • Th1 Cells
  • Repressor Proteins
  • Receptors, Chemokine
  • Receptors, CCR7
  • Proto-Oncogene Proteins c-jun
  • Phosphorylation
  • Phosphoproteins