Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis.

Publication ,  Journal Article
Ragione, FD; Cucciolla, V; Borriello, A; Pietra, VD; Pontoni, G; Racioppi, L; Manna, C; Galletti, P; Zappia, V
Published in: Biochem Biophys Res Commun
November 30, 2000

2-(3,4-Dihydroxyphenyl)ethanol (DPE), a naturally occurring phenolic antioxidant molecule found in olive oil, has been reported to exert several biological and pharmacological activities. We studied the effect of this compound on the proliferation and survival of HL60 cell line. Concentrations from 50 to 100 microM DPE, comparable to its olive oil content, caused a complete arrest of HL60 cell proliferation and the induction of apoptosis. This was demonstrated by flow cytometric analyses, poly(ADP-ribose) polymerase cleavage, and caspase 3 activation. The apoptotic effect requires the presence of two ortho-hydroxyl groups on the phenyl ring, since tyrosol, 2-(4-hydroxyphenyl)ethanol, did not induce either cell growth arrest or apoptosis. DPE-dependent apoptosis is associated with an early release of cytochrome c from mitochondria which precedes caspase 8 activation, thus ruling out the engagement of cell death receptors in the apoptotic process. 2-(3,4-Dihydroxyphenyl)ethanol induced cell death in quiescent and differentiated HL60 cells, as well as in resting and activated peripheral blood lymphocytes, while did not cause cell death in two colorectal cell lines (HT-29 and CaCo2). These results suggest that DPE down-regulates the immunological response, thus explaining the well-known antinflammatory and chemopreventive effects of olive oil at the intestinal level.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

November 30, 2000

Volume

278

Issue

3

Start / End Page

733 / 739

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Plant Oils
  • Phenylethyl Alcohol
  • Olive Oil
  • Kinetics
  • Humans
  • Homogentisic Acid
  • HL-60 Cells
  • Cytochrome c Group
  • Cholecalciferol
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ragione, F. D., Cucciolla, V., Borriello, A., Pietra, V. D., Pontoni, G., Racioppi, L., … Zappia, V. (2000). Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis. Biochem Biophys Res Commun, 278(3), 733–739. https://doi.org/10.1006/bbrc.2000.3875
Ragione, F. D., V. Cucciolla, A. Borriello, V. D. Pietra, G. Pontoni, L. Racioppi, C. Manna, P. Galletti, and V. Zappia. “Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis.Biochem Biophys Res Commun 278, no. 3 (November 30, 2000): 733–39. https://doi.org/10.1006/bbrc.2000.3875.
Ragione FD, Cucciolla V, Borriello A, Pietra VD, Pontoni G, Racioppi L, et al. Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis. Biochem Biophys Res Commun. 2000 Nov 30;278(3):733–9.
Ragione, F. D., et al. “Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis.Biochem Biophys Res Commun, vol. 278, no. 3, Nov. 2000, pp. 733–39. Pubmed, doi:10.1006/bbrc.2000.3875.
Ragione FD, Cucciolla V, Borriello A, Pietra VD, Pontoni G, Racioppi L, Manna C, Galletti P, Zappia V. Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis. Biochem Biophys Res Commun. 2000 Nov 30;278(3):733–739.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

November 30, 2000

Volume

278

Issue

3

Start / End Page

733 / 739

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Plant Oils
  • Phenylethyl Alcohol
  • Olive Oil
  • Kinetics
  • Humans
  • Homogentisic Acid
  • HL-60 Cells
  • Cytochrome c Group
  • Cholecalciferol