Skip to main content
Journal cover image

Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation.

Publication ,  Journal Article
Masci, AM; Paz, FL; Borriello, A; Cassano, S; Della Pietra, V; Stoiber, H; Matarese, G; Della Ragione, F; Zappacosta, S; Racioppi, L
Published in: Eur J Immunol
June 1999

The pathogenesis of the decline of CD4 lymphocyte counts accompanying the typical course of HIV-1 infection is not completely defined and might be related to a differential susceptibility of naive and memory cells to HIV-1 exposure. Here, we examined the effects induced by heat-inactivated HIV-1 virions on these lymphocyte populations. Exposure of CD45RA naive T cells to inactivated viral particles induced a marked decrease of both mitogenic responses and activation-induced apoptosis. Conversely, the growth of CD45RO cells was less severely restrained. Analysis of intracellular levels of cell cycle regulatory proteins revealed an arrest at the G1/S restriction point of the naive but not memory subset. This effect was associated with alterations in phosphotyrosine profile and with a marked decrease of ERK and NJK kinase activation. Finally, up-regulation of the cAMP-dependent protein kinase A (PKA) activity induced by mitogens was not affected by virus. Altogether, these findings show that interaction of HIV-1 with the T cell surface is sufficient to inhibit the proliferative response of the CD4CD45RA subset by disturbing proximal TCR signaling. This mechanism would affect renewal of naive lymphocytes, contributing in such a way to the impairment of T cell turnover during the course of HIV-1 infection.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

June 1999

Volume

29

Issue

6

Start / End Page

1879 / 1889

Location

Germany

Related Subject Headings

  • Signal Transduction
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Serine-Threonine Kinases
  • Phosphotyrosine
  • Lymphocyte Activation
  • Leukocyte Common Antigens
  • In Vitro Techniques
  • Immunology
  • Immunologic Memory
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Masci, A. M., Paz, F. L., Borriello, A., Cassano, S., Della Pietra, V., Stoiber, H., … Racioppi, L. (1999). Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation. Eur J Immunol, 29(6), 1879–1889. https://doi.org/10.1002/(SICI)1521-4141(199906)29:06<1879::AID-IMMU1879>3.0.CO;2-2
Masci, A. M., F. L. Paz, A. Borriello, S. Cassano, V. Della Pietra, H. Stoiber, G. Matarese, F. Della Ragione, S. Zappacosta, and L. Racioppi. “Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation.Eur J Immunol 29, no. 6 (June 1999): 1879–89. https://doi.org/10.1002/(SICI)1521-4141(199906)29:06<1879::AID-IMMU1879>3.0.CO;2-2.
Masci AM, Paz FL, Borriello A, Cassano S, Della Pietra V, Stoiber H, et al. Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation. Eur J Immunol. 1999 Jun;29(6):1879–89.
Masci, A. M., et al. “Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation.Eur J Immunol, vol. 29, no. 6, June 1999, pp. 1879–89. Pubmed, doi:10.1002/(SICI)1521-4141(199906)29:06<1879::AID-IMMU1879>3.0.CO;2-2.
Masci AM, Paz FL, Borriello A, Cassano S, Della Pietra V, Stoiber H, Matarese G, Della Ragione F, Zappacosta S, Racioppi L. Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation. Eur J Immunol. 1999 Jun;29(6):1879–1889.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

June 1999

Volume

29

Issue

6

Start / End Page

1879 / 1889

Location

Germany

Related Subject Headings

  • Signal Transduction
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Serine-Threonine Kinases
  • Phosphotyrosine
  • Lymphocyte Activation
  • Leukocyte Common Antigens
  • In Vitro Techniques
  • Immunology
  • Immunologic Memory
  • Humans