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Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow.

Publication ,  Journal Article
Olivier, LA; Yen, J; Reichert, WM; Truskey, GA
Published in: Biotechnology progress
January 1999

The manner in which fluid stresses are transmitted from the apical to the basal surface of the endothelium will influence the dynamics of cell/substrate contacts. Such dynamics could be important in the design of synthetic vascular grafts to promote endothelial cell adhesion. To examine whether the initial response of cell/substrate contact sites to flow depends on the magnitude of the applied shear stress, subconfluent monolayers of endothelial cells were exposed to flow at 10, 20, and 30 dyn cm-2 wall shear stresses for 20 min. Cell/substrate contact sites were visualized with total internal reflection fluorescence microscopy. Flow induced a rapid fluctuation in the membrane topography, which was reflected in dynamic changes in cell/substrate contacts. Exposure to flow caused marked changes in contact area. Contact movement occurred normal and parallel to the direction of flow. Contact sites demonstrated significant variability in contact area at 30 dyn cm-2 during the experiment but no significant movement of the contact sites in flow direction after 20 min of flow. Mean square displacements of the contact center of mass were described in terms of a directed diffusion model. Prior to onset of flow, contact movement was random. Flow induced a significant convective component to contact movement for 300-600 s, followed by reestablishment of diffusive growth and movement of contacts. These results suggest that fluid stresses are rapidly transmitted from the apical to the basal surface of the cell via the cytoskeleton.

Duke Scholars

Published In

Biotechnology progress

DOI

EISSN

1520-6033

ISSN

8756-7938

Publication Date

January 1999

Volume

15

Issue

1

Start / End Page

33 / 42

Related Subject Headings

  • Vascular Surgical Procedures
  • Stress, Mechanical
  • Endothelium, Vascular
  • Cells, Cultured
  • Cell Adhesion
  • Cattle
  • Biotechnology
  • Animals
  • 40 Engineering
  • 31 Biological sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Olivier, L. A., Yen, J., Reichert, W. M., & Truskey, G. A. (1999). Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow. Biotechnology Progress, 15(1), 33–42. https://doi.org/10.1021/bp980107e
Olivier, L. A., J. Yen, W. M. Reichert, and G. A. Truskey. “Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow.Biotechnology Progress 15, no. 1 (January 1999): 33–42. https://doi.org/10.1021/bp980107e.
Olivier LA, Yen J, Reichert WM, Truskey GA. Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow. Biotechnology progress. 1999 Jan;15(1):33–42.
Olivier, L. A., et al. “Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow.Biotechnology Progress, vol. 15, no. 1, Jan. 1999, pp. 33–42. Epmc, doi:10.1021/bp980107e.
Olivier LA, Yen J, Reichert WM, Truskey GA. Short-term cell/substrate contact dynamics of subconfluent endothelial cells following exposure to laminar flow. Biotechnology progress. 1999 Jan;15(1):33–42.
Journal cover image

Published In

Biotechnology progress

DOI

EISSN

1520-6033

ISSN

8756-7938

Publication Date

January 1999

Volume

15

Issue

1

Start / End Page

33 / 42

Related Subject Headings

  • Vascular Surgical Procedures
  • Stress, Mechanical
  • Endothelium, Vascular
  • Cells, Cultured
  • Cell Adhesion
  • Cattle
  • Biotechnology
  • Animals
  • 40 Engineering
  • 31 Biological sciences