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Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta.

Publication ,  Journal Article
Abdulovic, AL; Minesinger, BK; Jinks-Robertson, S
Published in: DNA Repair (Amst)
September 1, 2007

Translesion synthesis (TLS) DNA polymerases are specialized to bypass lesions that block replicative polymerases and prevent complete genome duplication. Current TLS models hypothesize that PCNA, the polymerase processivity clamp, is important for regulating the access and loading of the low fidelity TLS polymerases onto DNA in response to replication-blocking lesions. PCNA binds to the C-terminus of yeast Poleta, for example, and this interaction is required for cell survival after UV irradiation. Previously, we identified two spontaneous, Polzeta-dependent "complex" mutation hotspots using the lys2DeltaA746 frameshift reversion assay in repair-compromised cells. In the current study we observed an accumulation of Polzeta-dependent complex frameshifts at a third hotspot in Poleta-deficient cells. Interestingly, the sequence of this third hotspot is the reverse complement of the two hotspots previously identified, suggesting that the utilization of Polzeta and Poleta may be related to the position of the relevant lesion on either the leading- or lagging-strand template. Using the lys2DeltaA746 assay system, we investigated changes in the accumulation of complex events at hotspots when the direction of replication was reversed in repair-compromised cells with either wildtype Poleta, a deletion of Poleta, or a mutant of Poleta that cannot interact with PCNA. Our results suggest that there is a polymerase hierarchy between Poleta and Polzeta in the bypass of certain lesions and that the interaction of Poleta with PCNA is needed for some, but not all, spontaneous lesion bypass.

Duke Scholars

Published In

DNA Repair (Amst)

DOI

ISSN

1568-7864

Publication Date

September 1, 2007

Volume

6

Issue

9

Start / End Page

1307 / 1318

Location

Netherlands

Related Subject Headings

  • Saccharomyces cerevisiae
  • Proliferating Cell Nuclear Antigen
  • Molecular Sequence Data
  • Gene Expression Regulation, Fungal
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA, Fungal
  • DNA Replication
  • DNA Repair
  • DNA Damage
 

Citation

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ICMJE
MLA
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Abdulovic, A. L., Minesinger, B. K., & Jinks-Robertson, S. (2007). Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta. DNA Repair (Amst), 6(9), 1307–1318. https://doi.org/10.1016/j.dnarep.2007.02.026
Abdulovic, Amy L., Brenda K. Minesinger, and Sue Jinks-Robertson. “Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta.DNA Repair (Amst) 6, no. 9 (September 1, 2007): 1307–18. https://doi.org/10.1016/j.dnarep.2007.02.026.
Abdulovic AL, Minesinger BK, Jinks-Robertson S. Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta. DNA Repair (Amst). 2007 Sep 1;6(9):1307–18.
Abdulovic, Amy L., et al. “Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta.DNA Repair (Amst), vol. 6, no. 9, Sept. 2007, pp. 1307–18. Pubmed, doi:10.1016/j.dnarep.2007.02.026.
Abdulovic AL, Minesinger BK, Jinks-Robertson S. Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta. DNA Repair (Amst). 2007 Sep 1;6(9):1307–1318.
Journal cover image

Published In

DNA Repair (Amst)

DOI

ISSN

1568-7864

Publication Date

September 1, 2007

Volume

6

Issue

9

Start / End Page

1307 / 1318

Location

Netherlands

Related Subject Headings

  • Saccharomyces cerevisiae
  • Proliferating Cell Nuclear Antigen
  • Molecular Sequence Data
  • Gene Expression Regulation, Fungal
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA, Fungal
  • DNA Replication
  • DNA Repair
  • DNA Damage