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Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiae.

Publication ,  Journal Article
Minesinger, BK; Jinks-Robertson, S
Published in: Genetics
April 2005

DNA lesions that arise during normal cellular metabolism can block the progress of replicative DNA polymerases, leading to cell cycle arrest and, in higher eukaryotes, apoptosis. Alternatively, such blocking lesions can be temporarily tolerated using either a recombination- or a translesion synthesis-based bypass mechanism. In Saccharomyces cerevisiae, members of the RAD6 epistasis group are key players in the regulation of lesion bypass by the translesion DNA polymerase Polzeta. In this study, changes in the reversion rate and spectrum of the lys2DeltaA746 -1 frameshift allele have been used to evaluate how the loss of members of the RAD6 epistasis group affects Polzeta-dependent mutagenesis in response to spontaneous damage. Our data are consistent with a model in which Polzeta-dependent mutagenesis relies on the presence of either Rad5 or Rad18, which promote two distinct error-prone pathways that partially overlap with respect to lesion specificity. The smallest subunit of Poldelta, Pol32, is also required for Polzeta-dependent spontaneous mutagenesis, suggesting a cooperative role between Poldelta and Polzeta for the bypass of spontaneous lesions. A third error-free pathway relies on the presence of Mms2, but may not require PCNA.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

April 2005

Volume

169

Issue

4

Start / End Page

1939 / 1955

Location

United States

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Ubiquitin-Conjugating Enzymes
  • Saccharomyces cerevisiae Proteins
  • Protein Binding
  • Proliferating Cell Nuclear Antigen
  • Mutation
  • Mutagenesis
  • Monte Carlo Method
  • Molecular Sequence Data
  • Models, Genetic
 

Citation

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Minesinger, B. K., & Jinks-Robertson, S. (2005). Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiae. Genetics, 169(4), 1939–1955. https://doi.org/10.1534/genetics.104.033894
Minesinger, Brenda K., and Sue Jinks-Robertson. “Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiae.Genetics 169, no. 4 (April 2005): 1939–55. https://doi.org/10.1534/genetics.104.033894.
Minesinger, Brenda K., and Sue Jinks-Robertson. “Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiae.Genetics, vol. 169, no. 4, Apr. 2005, pp. 1939–55. Pubmed, doi:10.1534/genetics.104.033894.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

April 2005

Volume

169

Issue

4

Start / End Page

1939 / 1955

Location

United States

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Ubiquitin-Conjugating Enzymes
  • Saccharomyces cerevisiae Proteins
  • Protein Binding
  • Proliferating Cell Nuclear Antigen
  • Mutation
  • Mutagenesis
  • Monte Carlo Method
  • Molecular Sequence Data
  • Models, Genetic