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Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition.

Publication ,  Journal Article
Doyle, ME; Boggs, L; Attia, R; Cooper, LR; Saban, DR; Nguyen, CQ; Peck, AB
Published in: Am J Pathol
October 2007

Sjögren's syndrome (SjS) is a human autoimmune disease characterized by exocrine dysfunction resulting from chronic autoimmune attack primarily against the lacrimal and/or salivary glands. Although, we previously established a good correlation between SjS in humans and autoimmune exocrinopathy in NOD/LtJ mice an in-depth evaluation of lacrimal gland disease in the NOD/LtJ mouse has remained limited. This leaves a major gap in our understanding of the dacryoadenitis/keratoconjunctivitis sicca in this model. Here we characterize the development of the autoimmune dacryoadenitis in NOD/LtJ and NOD.B10-H2(b) mice in comparison with age- and sex-matched nonautoimmune CD1 mice. We observed a decline in tear production beginning at 8 weeks of age in both NOD/LtJ and NOD.B10-H2(b) mice, continuing throughout the 40 to 46 weeks studied. This correlated with a quantifiable increase in mixed T- and B-lymphocyte infiltrations in the extraorbital lacrimal glands. In addition, temporal differences in tear protein expression between NOD/LtJ and CD1 mice were identified using two-dimensional gel electrophoresis and tandem mass spectrometry. Thus, using this model we can identify potentially important pathophysiological mechanisms of the autoimmune attack and possible diagnostic markers for development of SjS-associated dacryoadenitis.

Duke Scholars

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

October 2007

Volume

171

Issue

4

Start / End Page

1224 / 1236

Location

United States

Related Subject Headings

  • Tears
  • Tandem Mass Spectrometry
  • T-Lymphocytes
  • Sex Factors
  • Pathology
  • Molecular Sequence Data
  • Mice, Inbred NOD
  • Mice
  • Male
  • Lacrimal Apparatus
 

Citation

APA
Chicago
ICMJE
MLA
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Doyle, M. E., Boggs, L., Attia, R., Cooper, L. R., Saban, D. R., Nguyen, C. Q., & Peck, A. B. (2007). Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition. Am J Pathol, 171(4), 1224–1236. https://doi.org/10.2353/ajpath.2007.070388
Doyle, Máire E., Lori Boggs, Robert Attia, Lauren R. Cooper, Daniel R. Saban, Cuong Q. Nguyen, and Ammon B. Peck. “Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition.Am J Pathol 171, no. 4 (October 2007): 1224–36. https://doi.org/10.2353/ajpath.2007.070388.
Doyle ME, Boggs L, Attia R, Cooper LR, Saban DR, Nguyen CQ, et al. Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition. Am J Pathol. 2007 Oct;171(4):1224–36.
Doyle, Máire E., et al. “Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition.Am J Pathol, vol. 171, no. 4, Oct. 2007, pp. 1224–36. Pubmed, doi:10.2353/ajpath.2007.070388.
Doyle ME, Boggs L, Attia R, Cooper LR, Saban DR, Nguyen CQ, Peck AB. Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition. Am J Pathol. 2007 Oct;171(4):1224–1236.
Journal cover image

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

October 2007

Volume

171

Issue

4

Start / End Page

1224 / 1236

Location

United States

Related Subject Headings

  • Tears
  • Tandem Mass Spectrometry
  • T-Lymphocytes
  • Sex Factors
  • Pathology
  • Molecular Sequence Data
  • Mice, Inbred NOD
  • Mice
  • Male
  • Lacrimal Apparatus