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Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation.

Publication ,  Journal Article
Terry-Lorenzo, RT; Roadcap, DW; Otsuka, T; Blanpied, TA; Zamorano, PL; Garner, CC; Shenolikar, S; Ehlers, MD
Published in: Mol Biol Cell
May 2005

The majority of excitatory synapses in the mammalian brain form on filopodia and spines, actin-rich membrane protrusions present on neuronal dendrites. The biochemical events that induce filopodia and remodel these structures into dendritic spines remain poorly understood. Here, we show that the neuronal actin- and protein phosphatase-1-binding protein, neurabin-I, promotes filopodia in neurons and nonneuronal cells. Neurabin-I actin-binding domain bundled F-actin, promoted filopodia, and delayed the maturation of dendritic spines in cultured hippocampal neurons. In contrast, dimerization of neurabin-I via C-terminal coiled-coil domains and association of protein phosphatase-1 (PP1) with neurabin-I through a canonical KIXF motif inhibited filopodia. Furthermore, the expression of a neurabin-I polypeptide unable to bind PP1 delayed the maturation of neuronal filopodia into spines, reduced the synaptic targeting of AMPA-type glutamate (GluR1) receptors, and decreased AMPA receptor-mediated synaptic transmission. Reduction of endogenous neurabin levels by interference RNA (RNAi)-mediated knockdown also inhibited the surface expression of GluR1 receptors. Together, our studies suggested that disrupting the functions of a cytoskeletal neurabin/PP1 complex enhanced filopodia and impaired surface GluR1 expression in hippocampal neurons, thereby hindering the morphological and functional maturation of dendritic spines.

Duke Scholars

Published In

Mol Biol Cell

DOI

ISSN

1059-1524

Publication Date

May 2005

Volume

16

Issue

5

Start / End Page

2349 / 2362

Location

United States

Related Subject Headings

  • Transfection
  • Synaptic Transmission
  • Recombinant Fusion Proteins
  • Receptors, AMPA
  • Rats
  • RNA Interference
  • Pseudopodia
  • Protein Structure, Tertiary
  • Protein Phosphatase 1
  • Phosphoprotein Phosphatases
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Terry-Lorenzo, R. T., Roadcap, D. W., Otsuka, T., Blanpied, T. A., Zamorano, P. L., Garner, C. C., … Ehlers, M. D. (2005). Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation. Mol Biol Cell, 16(5), 2349–2362. https://doi.org/10.1091/mbc.e04-12-1054
Terry-Lorenzo, Ryan T., David W. Roadcap, Takeshi Otsuka, Thomas A. Blanpied, Pedro L. Zamorano, Craig C. Garner, Shirish Shenolikar, and Michael D. Ehlers. “Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation.Mol Biol Cell 16, no. 5 (May 2005): 2349–62. https://doi.org/10.1091/mbc.e04-12-1054.
Terry-Lorenzo RT, Roadcap DW, Otsuka T, Blanpied TA, Zamorano PL, Garner CC, et al. Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation. Mol Biol Cell. 2005 May;16(5):2349–62.
Terry-Lorenzo, Ryan T., et al. “Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation.Mol Biol Cell, vol. 16, no. 5, May 2005, pp. 2349–62. Pubmed, doi:10.1091/mbc.e04-12-1054.
Terry-Lorenzo RT, Roadcap DW, Otsuka T, Blanpied TA, Zamorano PL, Garner CC, Shenolikar S, Ehlers MD. Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation. Mol Biol Cell. 2005 May;16(5):2349–2362.

Published In

Mol Biol Cell

DOI

ISSN

1059-1524

Publication Date

May 2005

Volume

16

Issue

5

Start / End Page

2349 / 2362

Location

United States

Related Subject Headings

  • Transfection
  • Synaptic Transmission
  • Recombinant Fusion Proteins
  • Receptors, AMPA
  • Rats
  • RNA Interference
  • Pseudopodia
  • Protein Structure, Tertiary
  • Protein Phosphatase 1
  • Phosphoprotein Phosphatases