Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.
Publication
, Journal Article
Shenoy, SK
Published in: Methods Mol Biol
2011
The heptahelical G protein-coupled receptors (GPCRs) receive and transmit a wide range of extracellular stimuli and induce a wide array of cellular responses by activating signaling kinases. It has become increasingly evident that the agonist-stimulated GPCR complexed with the adaptor protein, β-arrestin, serves as a focal point to recruit, activate, and target kinases to discrete subcellular compartments. This chapter describes a protocol to visualize the changes in the subcellular distribution of activated extracellular signal-regulated kinases 1 and 2 (ERK1/2) when induced by the angiotensin II type 1a receptor.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2011
Volume
756
Start / End Page
333 / 342
Location
United States
Related Subject Headings
- Signal Transduction
- Receptors, G-Protein-Coupled
- Receptor, Angiotensin, Type 1
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinase 1
- Microscopy, Fluorescence
- Humans
- Enzyme Activation
- Developmental Biology
- Cell Line
Citation
APA
Chicago
ICMJE
MLA
NLM
Shenoy, S. K. (2011). Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy. Methods Mol Biol, 756, 333–342. https://doi.org/10.1007/978-1-61779-160-4_20
Shenoy, Sudha K. “Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.” Methods Mol Biol 756 (2011): 333–42. https://doi.org/10.1007/978-1-61779-160-4_20.
Shenoy SK. Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy. Methods Mol Biol. 2011;756:333–42.
Shenoy, Sudha K. “Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.” Methods Mol Biol, vol. 756, 2011, pp. 333–42. Pubmed, doi:10.1007/978-1-61779-160-4_20.
Shenoy SK. Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy. Methods Mol Biol. 2011;756:333–342.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2011
Volume
756
Start / End Page
333 / 342
Location
United States
Related Subject Headings
- Signal Transduction
- Receptors, G-Protein-Coupled
- Receptor, Angiotensin, Type 1
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinase 1
- Microscopy, Fluorescence
- Humans
- Enzyme Activation
- Developmental Biology
- Cell Line