Scholars@Duke publication: Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons.

Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons.

Publication , Journal Article

Liu, L; Pugh, W; Ma, H; Simon, SA

Published in: Brain Res

July 16, 1993

Nicotinic acetylcholine receptors (nAChRs) were identified in a subpopulation of cultured adult rat trigeminal ganglia (TG) neurons by whole-cell patch-clamp recordings. Dimethylphenylpiperazinium (DMPP), a nAChR agonist, induced inward currents in 21/68 of TG neurons having soma diameters greater than 28 microns. These currents were inhibited by hexamethonium, mecamylamine and atropine, indicating the presence of neuronal ganglionic-type nAChRs. This interpretation is consistent with the finding that the nicotine- or DMPP-induced currents were not inhibited by alpha-bungarotoxin (alpha-Bng) in 5 of the 9 cells tested with this compound. However, in 2 of the 9 cells tested, the DMPP-induced currents were completely inhibited by alpha-Bng, and in the remaining two cells tested, the currents were partially inhibited by alpha-Bng. About 22% of the cells having diameters > or = 28 microns were specifically labeled with FITC-labeled alpha-Bng, whereas only 2% of the cells with soma diameters < 28 microns were labeled. These data taken together suggest that more than one subtype of nAChR is present in TG.

Duke Scholars

Author Sidney Arthur Simon Neurobiology

Published In

Brain Res

DOI

10.1016/0006-8993(93)90609-q

ISSN

0006-8993

Publication Date

July 16, 1993

Volume

617

Issue

Start / End Page

37 / 42

Location

Netherlands

Related Subject Headings

Trigeminal Ganglion
Receptors, Cholinergic
Rats, Sprague-Dawley
Rats
Neurology & Neurosurgery
Nerve Fibers
Membrane Potentials
Male
Ganglia, Spinal
Female

Citation

APA

Chicago

ICMJE

MLA

NLM

Liu, L., Pugh, W., Ma, H., & Simon, S. A. (1993). Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons. Brain Res, 617(1), 37–42. https://doi.org/10.1016/0006-8993(93)90609-q

Liu, L., W. Pugh, H. Ma, and S. A. Simon. “Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons.” Brain Res 617, no. 1 (July 16, 1993): 37–42. https://doi.org/10.1016/0006-8993(93)90609-q.

Liu L, Pugh W, Ma H, Simon SA. Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons. Brain Res. 1993 Jul 16;617(1):37–42.

Liu, L., et al. “Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons.” Brain Res, vol. 617, no. 1, July 1993, pp. 37–42. Pubmed, doi:10.1016/0006-8993(93)90609-q.

Liu L, Pugh W, Ma H, Simon SA. Identification of acetylcholine receptors in adult rat trigeminal ganglion neurons. Brain Res. 1993 Jul 16;617(1):37–42.