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S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology.

Publication ,  Journal Article
Sumida, GM; Stamer, WD
Published in: Am J Physiol Cell Physiol
May 2011

Elevated intraocular pressure is the main risk factor in primary open-angle glaucoma, involving an increased resistance to aqueous humor outflow in the juxtacanalicular region of the conventional outflow pathway which includes the trabecular meshwork (TM) and the inner wall of Schlemm's canal (SC). Previously, sphingosine-1-phosphate (S1P) was shown to decrease outflow facility in porcine and human eyes, thus increasing outflow resistance and intraocular pressure. Owing to S1P's known effect of increasing barrier function in endothelial cells and the robust expression of the S1P₁ receptor on the inner wall of SC, we hypothesized that S1P₁ receptor activation promotes junction formation and decreases outflow facility. The effects of subtype-specific S1P receptor compounds were tested in human and porcine whole-eye perfusions and human primary cultures of SC and TM cells to determine the receptor responsible for S1P effects on outflow resistance. The S1P₁-specific agonist SEW2871 failed to both mimic S1P effects in paired human eye perfusions, as well as increase myosin light chain (MLC) phosphorylation in cell culture, a prominent outcome in S1P-treated SC and TM cells. In contrast, the S1P₂ antagonist JTE-013, but not the S1P₁ or S1P₁,₃ antagonists, blocked the S1P-promoted increase in MLC phosphorylation. Moreover, JTE-013 prevented S1P-induced decrease in outflow facility in perfused human eyes (P < 0.05, n = 6 pairs). Similarly, porcine eyes perfused with JTE-013 + S1P did not differ from eyes with JTE-013 alone (P = 0.53, n = 3). These results demonstrate that S1P₂ , and not S1P₁ or S1P₃, receptor activation increases conventional outflow resistance and is a potential target to regulate intraocular pressure.

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Published In

Am J Physiol Cell Physiol

DOI

EISSN

1522-1563

Publication Date

May 2011

Volume

300

Issue

5

Start / End Page

C1164 / C1171

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Thiophenes
  • Swine
  • Sphingosine
  • Receptors, Lysosphingolipid
  • Pyridines
  • Pyrazoles
  • Physiology
  • Phosphorylation
  • Oxadiazoles
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sumida, G. M., & Stamer, W. D. (2011). S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology. Am J Physiol Cell Physiol, 300(5), C1164–C1171. https://doi.org/10.1152/ajpcell.00437.2010
Sumida, Grant M., and W Daniel Stamer. “S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology.Am J Physiol Cell Physiol 300, no. 5 (May 2011): C1164–71. https://doi.org/10.1152/ajpcell.00437.2010.
Sumida GM, Stamer WD. S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology. Am J Physiol Cell Physiol. 2011 May;300(5):C1164–71.
Sumida, Grant M., and W. Daniel Stamer. “S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology.Am J Physiol Cell Physiol, vol. 300, no. 5, May 2011, pp. C1164–71. Pubmed, doi:10.1152/ajpcell.00437.2010.
Sumida GM, Stamer WD. S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology. Am J Physiol Cell Physiol. 2011 May;300(5):C1164–C1171.

Published In

Am J Physiol Cell Physiol

DOI

EISSN

1522-1563

Publication Date

May 2011

Volume

300

Issue

5

Start / End Page

C1164 / C1171

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Thiophenes
  • Swine
  • Sphingosine
  • Receptors, Lysosphingolipid
  • Pyridines
  • Pyrazoles
  • Physiology
  • Phosphorylation
  • Oxadiazoles