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Outflow physiology of the mouse eye: pressure dependence and washout.

Publication ,  Journal Article
Lei, Y; Overby, DR; Boussommier-Calleja, A; Stamer, WD; Ethier, CR
Published in: Invest Ophthalmol Vis Sci
March 2011

PURPOSE: Mice are commonly used in glaucoma research, but relatively little is known about aqueous outflow dynamics in the species. To facilitate future use of the mouse as a model of aqueous humor outflow, several fundamental physiological parameters were measured in the mouse eye. METHODS: Eyes from adult mice of either sex (C57BL/6 background) were enucleated, cannulated with a 33-gauge needle, and perfused at constant pressure while inflow was continuously measured. RESULTS: At 8 mm Hg, total outflow facility (C(total)) was 0.022 ± 0.005 μL/min/mm Hg (all values mean ± SD; n = 21). The flow-pressure relationship was linear up to 35 mm Hg. The conventional outflow facility (C(conv)) was 0.0066 ± 0.0009 μL/min/mm Hg, and the unconventional outflow (F(u)) was 0.114 ± 0.019 μL/min, both measured at room temperature. At 8 mm Hg, 66% of the outflow was via the unconventional pathway. In a more than 2-hour-long perfusion at 8 mm Hg, the rate of facility change was 2.4% ± 5.4% (n = 11) of starting facility per hour. The ocular compliance (0.086 ± 0.017 μL/mm Hg; n = 5) was comparable to the compliance of the perfusion system (0.100 ± 0.004 μL/mm Hg). CONCLUSIONS: Mouse eyes are similar to human eyes, in that they have no detectable washout rate and a linear pressure-flow relationship over a broad range of intraocular pressures. Because of the absence of washout and the apparent presence of a true Schlemm's canal, the mouse is a useful model for studying the physiology of the inner wall of Schlemm's canal and the conventional outflow tissues.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

March 2011

Volume

52

Issue

3

Start / End Page

1865 / 1871

Location

United States

Related Subject Headings

  • Ophthalmology & Optometry
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Intraocular Pressure
  • Female
  • Compliance
  • Blood-Aqueous Barrier
  • Aqueous Humor
  • Anterior Eye Segment
 

Citation

APA
Chicago
ICMJE
MLA
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Lei, Y., Overby, D. R., Boussommier-Calleja, A., Stamer, W. D., & Ethier, C. R. (2011). Outflow physiology of the mouse eye: pressure dependence and washout. Invest Ophthalmol Vis Sci, 52(3), 1865–1871. https://doi.org/10.1167/iovs.10-6019
Lei, Yuan, Darryl R. Overby, Alexandra Boussommier-Calleja, W Daniel Stamer, and C Ross Ethier. “Outflow physiology of the mouse eye: pressure dependence and washout.Invest Ophthalmol Vis Sci 52, no. 3 (March 2011): 1865–71. https://doi.org/10.1167/iovs.10-6019.
Lei Y, Overby DR, Boussommier-Calleja A, Stamer WD, Ethier CR. Outflow physiology of the mouse eye: pressure dependence and washout. Invest Ophthalmol Vis Sci. 2011 Mar;52(3):1865–71.
Lei, Yuan, et al. “Outflow physiology of the mouse eye: pressure dependence and washout.Invest Ophthalmol Vis Sci, vol. 52, no. 3, Mar. 2011, pp. 1865–71. Pubmed, doi:10.1167/iovs.10-6019.
Lei Y, Overby DR, Boussommier-Calleja A, Stamer WD, Ethier CR. Outflow physiology of the mouse eye: pressure dependence and washout. Invest Ophthalmol Vis Sci. 2011 Mar;52(3):1865–1871.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

March 2011

Volume

52

Issue

3

Start / End Page

1865 / 1871

Location

United States

Related Subject Headings

  • Ophthalmology & Optometry
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Intraocular Pressure
  • Female
  • Compliance
  • Blood-Aqueous Barrier
  • Aqueous Humor
  • Anterior Eye Segment