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Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1.

Publication ,  Journal Article
Heimark, RL; Kaochar, S; Stamer, WD
Published in: Curr Eye Res
November 2002

Purpose. The majority of resistance to outflow of aqueous humor resides at or near the inner wall of Schlemm's canal (SC). Transmembrane proteins that contribute to the generation of resistance to aqueous outflow likely participate in junctional complexes between SC cells. The purpose of the present study was to examine the expression of cadherins in SC cells that play a significant role in adherens junction complexes that control permeability of vascular endothelia. Methods. Identification of cadherin subtype mRNAs was examined by hybridization screening of three different SC cDNA libraries and by polymerase chain reaction analysis with degenerate primers. Expression of endothelial adherens proteins, vascular endothelial (VE)-cadherin and platelet endothelial cell adhesion molecule-1 (PECAM-1), was examined by western blot analyses of whole cell lysates prepared from SC and trabecular meshwork cells and by immunofluorescence microscopy of frozen sections of human anterior chambers. As controls, bovine retinal, bovine aortic, human umbilical vein and human iliac vein endothelial cells were examined for VE-cadherin expression. Results. Screens of SC cDNAs revealed abundant expression of N-cadherin and VE-cadherin. Expression of VE-cadherin protein was identified in both inner and outer wall SC cells, appropriately localized to SC intercellular borders and appeared as a single band of approximately 130 kDa by western blot analysis. Specific labeling of PECAM-1 was similar to VE-cadherin and appeared as a single band of approximately 130 kDa by western blot analysis. Conclusions. VE-cadherin and PECAM-1 expression in SC suggests that SC cells are vascular in origin and contain adherens protein likely involved in restricting fluid flow across the inner wall of SC.

Duke Scholars

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

November 2002

Volume

25

Issue

5

Start / End Page

299 / 308

Location

England

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA
  • Protein Binding
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Membrane Proteins
  • Humans
  • Gene Expression
  • Fluorescent Antibody Technique
 

Citation

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ICMJE
MLA
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Heimark, R. L., Kaochar, S., & Stamer, W. D. (2002). Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1. Curr Eye Res, 25(5), 299–308. https://doi.org/10.1076/ceyr.25.5.299.13495
Heimark, Ronald L., Salma Kaochar, and W Daniel Stamer. “Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1.Curr Eye Res 25, no. 5 (November 2002): 299–308. https://doi.org/10.1076/ceyr.25.5.299.13495.
Heimark RL, Kaochar S, Stamer WD. Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1. Curr Eye Res. 2002 Nov;25(5):299–308.
Heimark, Ronald L., et al. “Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1.Curr Eye Res, vol. 25, no. 5, Nov. 2002, pp. 299–308. Pubmed, doi:10.1076/ceyr.25.5.299.13495.
Heimark RL, Kaochar S, Stamer WD. Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1. Curr Eye Res. 2002 Nov;25(5):299–308.
Journal cover image

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

November 2002

Volume

25

Issue

5

Start / End Page

299 / 308

Location

England

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA
  • Protein Binding
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Membrane Proteins
  • Humans
  • Gene Expression
  • Fluorescent Antibody Technique