Scholars@Duke publication: Transthyretin sequesters amyloid beta protein and prevents amyloid formation.

Transthyretin sequesters amyloid beta protein and prevents amyloid formation.

Publication , Journal Article

Schwarzman, AL; Gregori, L; Vitek, MP; Lyubski, S; Strittmatter, WJ; Enghilde, JJ; Bhasin, R; Silverman, J; Weisgraber, KH; Coyle, PK

Published in: Proc Natl Acad Sci U S A

August 30, 1994

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The cardinal pathological features of Alzheimer disease are depositions of aggregated amyloid beta protein (A beta) in the brain and cerebrovasculature. However, the A beta is found in a soluble form in cerebrospinal fluid in healthy individuals and patients with Alzheimer disease. We postulate that sequestration of A beta precludes amyloid formation. Failure to sequester A beta in Alzheimer disease may result in amyloidosis. When we added A beta to cerebrospinal fluid of patients and controls it was rapidly sequestered into stable complexes with transthyretin. Complexes with apolipoprotein E, which has been shown to bind A beta in vitro, were not observed in cerebrospinal fluid. Additional in vitro studies showed that both purified transthyretin and apolipoprotein E prevent amyloid formation.

Duke Scholars

Author Michael P. Vitek Neurology, Behavioral Neurology

Author Warren James Strittmatter Neurology, Behavioral Neurology

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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.91.18.8368

ISSN

0027-8424

Publication Date

August 30, 1994

Volume

Issue

Start / End Page

8368 / 8372

Location

United States

Related Subject Headings

Protein Binding
Prealbumin
Molecular Sequence Data
Models, Molecular
In Vitro Techniques
Humans
Computer Graphics
Amyloid beta-Peptides
Amyloid Neuropathies
Amino Acid Sequence

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MLA

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Schwarzman, A. L., Gregori, L., Vitek, M. P., Lyubski, S., Strittmatter, W. J., Enghilde, J. J., … Coyle, P. K. (1994). Transthyretin sequesters amyloid beta protein and prevents amyloid formation. Proc Natl Acad Sci U S A, 91(18), 8368–8372. https://doi.org/10.1073/pnas.91.18.8368

Schwarzman, A. L., L. Gregori, M. P. Vitek, S. Lyubski, W. J. Strittmatter, J. J. Enghilde, R. Bhasin, J. Silverman, K. H. Weisgraber, and P. K. Coyle. “Transthyretin sequesters amyloid beta protein and prevents amyloid formation.” Proc Natl Acad Sci U S A 91, no. 18 (August 30, 1994): 8368–72. https://doi.org/10.1073/pnas.91.18.8368.

Schwarzman AL, Gregori L, Vitek MP, Lyubski S, Strittmatter WJ, Enghilde JJ, et al. Transthyretin sequesters amyloid beta protein and prevents amyloid formation. Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8368–72.

Schwarzman, A. L., et al. “Transthyretin sequesters amyloid beta protein and prevents amyloid formation.” Proc Natl Acad Sci U S A, vol. 91, no. 18, Aug. 1994, pp. 8368–72. Pubmed, doi:10.1073/pnas.91.18.8368.

Schwarzman AL, Gregori L, Vitek MP, Lyubski S, Strittmatter WJ, Enghilde JJ, Bhasin R, Silverman J, Weisgraber KH, Coyle PK. Transthyretin sequesters amyloid beta protein and prevents amyloid formation. Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8368–8372.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.91.18.8368

ISSN

0027-8424

Publication Date

August 30, 1994

Volume

Issue

Start / End Page

8368 / 8372

Location

United States

Related Subject Headings

Protein Binding
Prealbumin
Molecular Sequence Data
Models, Molecular
In Vitro Techniques
Humans
Computer Graphics
Amyloid beta-Peptides
Amyloid Neuropathies
Amino Acid Sequence