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Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling.

Publication ,  Journal Article
Christian, NA; Milone, MC; Ranka, SS; Li, G; Frail, PR; Davis, KP; Bates, FS; Therien, MJ; Ghoroghchian, PP; June, CH; Hammer, DA
Published in: Bioconjugate chemistry
January 2007

Dendritic cells (DCs) play a pivotal role in both immune tolerance and the initiation of immunological responses. The ability to track DCs in vivo is imperative for the development of DC-based cellular therapies and to advance our understanding of DC function and pathophysiology. Here, we conjugate a cell permeable peptide, Tat, to near-infrared (NIR) emissive polymersomes in order to enable efficient intracellular delivery for future DC tracking with these optical probes. NIR imaging allows quantitative, repetitive, in vivo detection of fluorophore-laden cells, at centimeter tissue depths without disturbing cellular function. Flow cytometry and confocal microscopy results indicate that Tat-mediated polymersome delivery to DCs is concentration and time dependent, resulting in punctate intracellular localization. Further, loading cells with Tat NIR emissive polymersomes does not interfere with cytokine-induced DC maturation and has modest effects on DC viability, but has a significant effect on mature DC-induced activation of naive T cells. We observe significant uptake of NIR emissive polymersomes when conjugated to the peptide, with a lower detection limit of 5000 labeled DCs. The extent of polymersome delivery is estimated as 70 000 +/- 10 000 vesicles/cell, equivalent to 0.7 +/- 0.1 fmol of NIR fluorophore. Our studies will enable future in vivo tracking of ex vivo labeled DCs by NIR fluorescence based imaging.

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Published In

Bioconjugate chemistry

DOI

EISSN

1520-4812

ISSN

1043-1802

Publication Date

January 2007

Volume

18

Issue

1

Start / End Page

31 / 40

Related Subject Headings

  • Time Factors
  • Spectroscopy, Near-Infrared
  • Phenotype
  • Organic Chemistry
  • Molecular Structure
  • Microscopy, Electron, Transmission
  • Lymphocyte Culture Test, Mixed
  • Humans
  • Gene Products, tat
  • Dendritic Cells
 

Citation

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Christian, N. A., Milone, M. C., Ranka, S. S., Li, G., Frail, P. R., Davis, K. P., … Hammer, D. A. (2007). Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling. Bioconjugate Chemistry, 18(1), 31–40. https://doi.org/10.1021/bc0601267
Christian, Natalie A., Michael C. Milone, Shraddha S. Ranka, Guizhi Li, Paul R. Frail, Kevin P. Davis, Frank S. Bates, et al. “Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling.Bioconjugate Chemistry 18, no. 1 (January 2007): 31–40. https://doi.org/10.1021/bc0601267.
Christian NA, Milone MC, Ranka SS, Li G, Frail PR, Davis KP, et al. Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling. Bioconjugate chemistry. 2007 Jan;18(1):31–40.
Christian, Natalie A., et al. “Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling.Bioconjugate Chemistry, vol. 18, no. 1, Jan. 2007, pp. 31–40. Epmc, doi:10.1021/bc0601267.
Christian NA, Milone MC, Ranka SS, Li G, Frail PR, Davis KP, Bates FS, Therien MJ, Ghoroghchian PP, June CH, Hammer DA. Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling. Bioconjugate chemistry. 2007 Jan;18(1):31–40.
Journal cover image

Published In

Bioconjugate chemistry

DOI

EISSN

1520-4812

ISSN

1043-1802

Publication Date

January 2007

Volume

18

Issue

1

Start / End Page

31 / 40

Related Subject Headings

  • Time Factors
  • Spectroscopy, Near-Infrared
  • Phenotype
  • Organic Chemistry
  • Molecular Structure
  • Microscopy, Electron, Transmission
  • Lymphocyte Culture Test, Mixed
  • Humans
  • Gene Products, tat
  • Dendritic Cells