Skip to main content

Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids.

Publication ,  Journal Article
Toffaletti, DL; Darrow, TL; Scott, DW
Published in: J Immunol
June 1983

Hybrid cell lines were established from fusions between lipopolysaccharide- (LPS) stimulated C57BL/6J spleen cells and MPC-11 tumor cells (45.6TG1.7, abbreviated M45), and were tested for their ability to immunize semiallogeneic mice against a parental tumor challenge. These hybrids were tumorigenic in syngeneic (BALB/c X C57BL/6J) F1 (CB6F1) mice but did not grow in semiallogeneic (BALB/c X A/J) F1 (CAF1) mice. All hybrids express both parental major histocompatibility antigens (H-2b and H-2d) as detected by indirect immunofluorescence and by their ability to function as either stimulators or targets for allogeneic cytotoxic lymphocytes (CTL). M45 tumor-associated antigens (TAA) were expressed on the hybrid surface as shown by their ability to act as either stimulators or targets for syngeneic CTL specific for M45 TAA. Immunization of semiallogeneic CAF1 mice with the hybrids i.p. followed by a challenge with M45 tumor cells resulted in extended survival when compared to untreated mice or animals immunized i.p. with M45 tumor cells. This immunity was specific and was not due to an allogeneic effect; immunization with an unrelated H-2bd tumor, 70Z/3, or H-2bd B6D2F1 spleen cells or with semiallogeneic spleen cells plus M45 did not protect mice from M45 challenge. Interestingly, prophylactic priming with semiallogeneic hybrid tumor cells or parental myeloma cells led to M45-specific CTL and "help" for an in vitro CTL response; however, the degree of CTL priming by hybrid tumors was not augmented when compared to the level of CTL achieved with parental tumor alone. Hence, stimulation of CTL activity per se by hybrid tumor cells cannot explain the protective effect of hybrid tumor immunization. These studies nevertheless confirm that semiallogeneic hybrids, which we show express TAA and alloantigens, can be used to immunize mice against a lethal syngeneic myeloma tumor challenge.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

June 1983

Volume

130

Issue

6

Start / End Page

2982 / 2986

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Plasmacytoma
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred A
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Toffaletti, D. L., Darrow, T. L., & Scott, D. W. (1983). Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids. J Immunol, 130(6), 2982–2986.
Toffaletti, D. L., T. L. Darrow, and D. W. Scott. “Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids.J Immunol 130, no. 6 (June 1983): 2982–86.
Toffaletti DL, Darrow TL, Scott DW. Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids. J Immunol. 1983 Jun;130(6):2982–6.
Toffaletti, D. L., et al. “Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids.J Immunol, vol. 130, no. 6, June 1983, pp. 2982–86.
Toffaletti DL, Darrow TL, Scott DW. Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids. J Immunol. 1983 Jun;130(6):2982–2986.

Published In

J Immunol

ISSN

0022-1767

Publication Date

June 1983

Volume

130

Issue

6

Start / End Page

2982 / 2986

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Plasmacytoma
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred A
  • Mice
  • Male
  • Lymphocyte Activation
  • Immunology