Skip to main content

Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers.

Publication ,  Journal Article
Toffaletti, J; Bird, C; Berg, C; Abrams, B
Published in: Clin Chem
August 1986

To determine if bias between different ionized calcium analyzers could be decreased, we analyzed 10 control fluids during a study in which ionized calcium was measured in more than 150 serum and whole-blood samples. After calibrating three ionized calcium analyzers (Radiometer ICA 1, Nova 8, and AVL 980) with the manufacturers' respective calibrators, we used the between-instrument differences of the control fluids to simulate recalibration of the analyzers during each analytical run. A filtered human serum pool containing ionized calcium at 1 mmol/L concentration, with CO2 removed and having no added buffer, was the only material that consistently decreased between-analyzer bias of both serum and whole blood. Another human serum pool containing about 1.3 mmol of ionized calcium and about 10 mmol of bicarbonate per liter was even better at minimizing analyzer biases for serum samples, but was not as effective for whole-blood samples. Some additives used to buffer pH apparently adversely affected both the accuracy and precision of some, but not other, calcium ion electrodes. We conclude that if a reference material is developed for calibration of ionized calcium analyzers, it should be tested on several analyzers for use with both serum and whole blood, and it should be at least as effective as a human serum material, such as that used here.

Duke Scholars

Published In

Clin Chem

ISSN

0009-9147

Publication Date

August 1986

Volume

32

Issue

8

Start / End Page

1548 / 1550

Location

England

Related Subject Headings

  • Reference Standards
  • Hydrogen-Ion Concentration
  • Humans
  • General Clinical Medicine
  • Calibration
  • Calcium
  • 3205 Medical biochemistry and metabolomics
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
  • 1101 Medical Biochemistry and Metabolomics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Toffaletti, J., Bird, C., Berg, C., & Abrams, B. (1986). Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers. Clin Chem, 32(8), 1548–1550.
Toffaletti, J., C. Bird, C. Berg, and B. Abrams. “Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers.Clin Chem 32, no. 8 (August 1986): 1548–50.
Toffaletti J, Bird C, Berg C, Abrams B. Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers. Clin Chem. 1986 Aug;32(8):1548–50.
Toffaletti, J., et al. “Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers.Clin Chem, vol. 32, no. 8, Aug. 1986, pp. 1548–50.
Toffaletti J, Bird C, Berg C, Abrams B. Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers. Clin Chem. 1986 Aug;32(8):1548–1550.

Published In

Clin Chem

ISSN

0009-9147

Publication Date

August 1986

Volume

32

Issue

8

Start / End Page

1548 / 1550

Location

England

Related Subject Headings

  • Reference Standards
  • Hydrogen-Ion Concentration
  • Humans
  • General Clinical Medicine
  • Calibration
  • Calcium
  • 3205 Medical biochemistry and metabolomics
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
  • 1101 Medical Biochemistry and Metabolomics