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Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding.

Publication ,  Journal Article
Truskey, GA; Herrmann, RA; Kait, J; Barber, KM
Published in: Arteriosclerosis, thrombosis, and vascular biology
February 1999

We tested the hypotheses that vascular cell adhesion molecule-1 (VCAM-1) expression on endothelium at lesion-prone sites in the rabbit aorta correlates with exposure to plasma cholesterol and that macrophage accumulation is associated with endothelial cells expressing VCAM-1. After rabbits were fed 0.25% cholesterol for 2 weeks, VCAM-1 expression was selectively increased at the distal and lateral portions of the major abdominal branches. In the arch and the celiac, superior mesenteric, and renal artery branches, VCAM-1 expression was positively correlated with the plasma cholesterol integrated over the duration of the experiments. After 2 weeks of cholesterol feeding, more macrophages were present around distal and lateral portions of the intercostal arteries and major abdominal branches relative to nonbranch regions. In the arch and around the intercostals and major abdominal branches, macrophage densities were positively correlated with the integrated plasma cholesterol. VCAM-1 and macrophage levels were correlated in lesion-prone regions. In normocholesterolemic rabbits, 23+/-4% (mean+/-SEM) of the macrophages were directly associated with VCAM-1-positive endothelium. After 2 weeks of 0.25% cholesterol feeding, the association increased to 37+/-4% (P<0.015). Associations were highest around the lateral and distal regions of the major abdominal branches. These results suggest that (1) VCAM-1 expression and intimal macrophage densities are influenced by plasma cholesterol and regional factors such as arterial fluid dynamics and (2) VCAM-1 plays a significant role in the localization of macrophages.

Duke Scholars

Published In

Arteriosclerosis, thrombosis, and vascular biology

DOI

EISSN

1524-4636

ISSN

1079-5642

Publication Date

February 1999

Volume

19

Issue

2

Start / End Page

393 / 401

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Tunica Intima
  • Time Factors
  • Rabbits
  • Osmolar Concentration
  • Male
  • Macrophages
  • Endothelium, Vascular
  • Disease Susceptibility
  • Cholesterol, Dietary
 

Citation

APA
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MLA
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Truskey, G. A., Herrmann, R. A., Kait, J., & Barber, K. M. (1999). Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding. Arteriosclerosis, Thrombosis, and Vascular Biology, 19(2), 393–401. https://doi.org/10.1161/01.atv.19.2.393
Truskey, G. A., R. A. Herrmann, J. Kait, and K. M. Barber. “Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding.Arteriosclerosis, Thrombosis, and Vascular Biology 19, no. 2 (February 1999): 393–401. https://doi.org/10.1161/01.atv.19.2.393.
Truskey GA, Herrmann RA, Kait J, Barber KM. Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding. Arteriosclerosis, thrombosis, and vascular biology. 1999 Feb;19(2):393–401.
Truskey, G. A., et al. “Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding.Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 19, no. 2, Feb. 1999, pp. 393–401. Epmc, doi:10.1161/01.atv.19.2.393.
Truskey GA, Herrmann RA, Kait J, Barber KM. Focal increases in vascular cell adhesion molecule-1 and intimal macrophages at atherosclerosis-susceptible sites in the rabbit aorta after short-term cholesterol feeding. Arteriosclerosis, thrombosis, and vascular biology. 1999 Feb;19(2):393–401.

Published In

Arteriosclerosis, thrombosis, and vascular biology

DOI

EISSN

1524-4636

ISSN

1079-5642

Publication Date

February 1999

Volume

19

Issue

2

Start / End Page

393 / 401

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Tunica Intima
  • Time Factors
  • Rabbits
  • Osmolar Concentration
  • Male
  • Macrophages
  • Endothelium, Vascular
  • Disease Susceptibility
  • Cholesterol, Dietary