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S-allele diversity in a natural population of Physalis crassifolia (Solanaceae) (ground cherry) assessed by RT-PCR.

Publication ,  Journal Article
Richman, AD; Uyenoyama, MK; Kohn, JR
Published in: Heredity
May 1996

Allelic diversity at the self-incompatibility (S-) locus in the ground cherry, Physalis crassifolia (Solanaceae), was surveyed in a natural population occurring in Deep Canyon, CA, using a molecular assay to determine the genotype of individual plants. A total of 28 different S-alleles were identified and sequenced from a sample of 22 plants. All plants examined were heterozygous, as expected under gametophytic self-incompatibility (GSI). The estimated number of alleles in this population is 43-44, comparable to allelic diversity reported for other species, as determined by the standard diallel crossing method. Allele frequencies in the sample deviated from the expectation of equal frequency under GSI; it is suggested that this deviation may result from sampling of related individuals. Molecular analysis of genotypes within single pollen donor families indicates that, for all alleles examined, segregation is consistent with predictions for single-locus GSI. The implications of a reliable and efficient molecular assay for determining the S-genotype of plants are discussed.

Duke Scholars

Published In

Heredity

DOI

EISSN

1365-2540

ISSN

0018-067X

Publication Date

May 1996

Volume

76 ( Pt 5)

Start / End Page

497 / 505

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Polymorphism, Restriction Fragment Length
  • Polymerase Chain Reaction
  • Plants
  • Molecular Sequence Data
  • Genotype
  • Genetic Variation
  • Evolutionary Biology
  • DNA, Plant
  • DNA, Complementary
 

Citation

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Richman, A. D., Uyenoyama, M. K., & Kohn, J. R. (1996). S-allele diversity in a natural population of Physalis crassifolia (Solanaceae) (ground cherry) assessed by RT-PCR. Heredity, 76 ( Pt 5), 497–505. https://doi.org/10.1038/hdy.1996.72
Richman, A. D., M. K. Uyenoyama, and J. R. Kohn. “S-allele diversity in a natural population of Physalis crassifolia (Solanaceae) (ground cherry) assessed by RT-PCR.Heredity 76 ( Pt 5) (May 1996): 497–505. https://doi.org/10.1038/hdy.1996.72.
Richman, A. D., et al. “S-allele diversity in a natural population of Physalis crassifolia (Solanaceae) (ground cherry) assessed by RT-PCR.Heredity, vol. 76 ( Pt 5), May 1996, pp. 497–505. Epmc, doi:10.1038/hdy.1996.72.

Published In

Heredity

DOI

EISSN

1365-2540

ISSN

0018-067X

Publication Date

May 1996

Volume

76 ( Pt 5)

Start / End Page

497 / 505

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Polymorphism, Restriction Fragment Length
  • Polymerase Chain Reaction
  • Plants
  • Molecular Sequence Data
  • Genotype
  • Genetic Variation
  • Evolutionary Biology
  • DNA, Plant
  • DNA, Complementary