Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.
Publication
, Journal Article
Velkey, JM; O'Shea, KS
Published in: Genesis
September 2003
We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express small stem-loop RNA transcripts corresponding to Oct4 mRNA. Cells transfected with Oct4 RNAi constructs demonstrated reduced levels of Oct4 mRNA and exhibited characteristics of trophectodermal differentiation. These findings support the critical role of Oct4 in regulating stem cell identity and suggest that future experiments using RNAi in ES cells can elucidate the roles of other genes affecting lineage specification during differentiation.
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Published In
Genesis
DOI
ISSN
1526-954X
Publication Date
September 2003
Volume
37
Issue
1
Start / End Page
18 / 24
Location
United States
Related Subject Headings
- Transfection
- Transcription Factors
- Time Factors
- Stem Cells
- Reverse Transcriptase Polymerase Chain Reaction
- RNA Polymerase III
- RNA Interference
- Promoter Regions, Genetic
- Plasmids
- Oligonucleotides, Antisense
Citation
APA
Chicago
ICMJE
MLA
NLM
Velkey, J. M., & O’Shea, K. S. (2003). Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. Genesis, 37(1), 18–24. https://doi.org/10.1002/gene.10218
Velkey, J Matthew, and K Sue O’Shea. “Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.” Genesis 37, no. 1 (September 2003): 18–24. https://doi.org/10.1002/gene.10218.
Velkey JM, O’Shea KS. Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. Genesis. 2003 Sep;37(1):18–24.
Velkey, J. Matthew, and K. Sue O’Shea. “Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.” Genesis, vol. 37, no. 1, Sept. 2003, pp. 18–24. Pubmed, doi:10.1002/gene.10218.
Velkey JM, O’Shea KS. Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. Genesis. 2003 Sep;37(1):18–24.
Published In
Genesis
DOI
ISSN
1526-954X
Publication Date
September 2003
Volume
37
Issue
1
Start / End Page
18 / 24
Location
United States
Related Subject Headings
- Transfection
- Transcription Factors
- Time Factors
- Stem Cells
- Reverse Transcriptase Polymerase Chain Reaction
- RNA Polymerase III
- RNA Interference
- Promoter Regions, Genetic
- Plasmids
- Oligonucleotides, Antisense