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Differential expression of gamma-aminobutyric acidA receptor subunits.

Publication ,  Journal Article
Garrett, KM; Saito, N; Duman, RS; Abel, MS; Ashton, RA; Fujimori, S; Beer, B; Tallman, JF; Vitek, MP; Blume, AJ
Published in: Mol Pharmacol
May 1990

A 1.8-kilobase (kb) cDNA clone for a beta 1 subunit of the human gamma-aminobutyric acidA (GABAA) receptor has been isolated and sequenced. The longest open reading frame of the clone, pCLL610, contains nucleotide sequence encoding a portion of the putative signal sequence followed by 449 amino acids of the entire mature protein. The deduced amino acid sequence of pCLL610 differs from a recently described human beta 1 subunit by a single amino acid. The amino acid sequences of the human GABAA receptor beta 1 subunits share 98% identity with the beta 1 subunits of the bovine and rat GABAA receptor, with the majority of the differences occurring in the intracellular loop between the M3 and M4 transmembrane regions of the protein. A single 11-kb transcript is observed in Northern blots of poly(A)+ RNA isolated from rat brain probed with nick-translated pCLL610. In human brain, the pCLL610 probe recognized the 11-kb message, in addition to two other bands between 8 and 11-kb. Developmental studies of rat brain mRNA show that the message of the GABAA beta 1 subunit is highest at birth, rapidly decreases, and reaches adult levels of 5 to 7 days of age. This is in contrast to the development of the alpha 1 subunit, which is low from days 1 to 5 and increases to adult levels by days 14 to 25. Relative levels of the mRNA for the alpha 1 and beta 1 subunits vary among rat brain regions. The levels of mRNA for the alpha 1 subunit are similar in the cortex, hippocampus, and midbrain, whereas cerebellar levels are twice those in the cortex. The rank order of the relative amount of beta 1 subunit message is hippocampus greater than cortex = midbrain greater than cerebellum. These data, taken with our previous study of the alpha 1 subunits of the GABAA receptor, suggest that the differences in the distribution and regulation of the alpha 1 and beta 1 subunits may reflect a variety of subunit combinations forming the GABAA receptor. Heterogeneity in the GABAA receptor composition may provide a molecular basis for the diverse pharmacological properties associated with this receptor.

Duke Scholars

Published In

Mol Pharmacol

ISSN

0026-895X

Publication Date

May 1990

Volume

37

Issue

5

Start / End Page

652 / 657

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Receptors, GABA-A
  • Rats
  • RNA, Messenger
  • Pharmacology & Pharmacy
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Macromolecular Substances
  • Humans
  • Gene Library
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Garrett, K. M., Saito, N., Duman, R. S., Abel, M. S., Ashton, R. A., Fujimori, S., … Blume, A. J. (1990). Differential expression of gamma-aminobutyric acidA receptor subunits. Mol Pharmacol, 37(5), 652–657.
Garrett, K. M., N. Saito, R. S. Duman, M. S. Abel, R. A. Ashton, S. Fujimori, B. Beer, J. F. Tallman, M. P. Vitek, and A. J. Blume. “Differential expression of gamma-aminobutyric acidA receptor subunits.Mol Pharmacol 37, no. 5 (May 1990): 652–57.
Garrett KM, Saito N, Duman RS, Abel MS, Ashton RA, Fujimori S, et al. Differential expression of gamma-aminobutyric acidA receptor subunits. Mol Pharmacol. 1990 May;37(5):652–7.
Garrett, K. M., et al. “Differential expression of gamma-aminobutyric acidA receptor subunits.Mol Pharmacol, vol. 37, no. 5, May 1990, pp. 652–57.
Garrett KM, Saito N, Duman RS, Abel MS, Ashton RA, Fujimori S, Beer B, Tallman JF, Vitek MP, Blume AJ. Differential expression of gamma-aminobutyric acidA receptor subunits. Mol Pharmacol. 1990 May;37(5):652–657.

Published In

Mol Pharmacol

ISSN

0026-895X

Publication Date

May 1990

Volume

37

Issue

5

Start / End Page

652 / 657

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Receptors, GABA-A
  • Rats
  • RNA, Messenger
  • Pharmacology & Pharmacy
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Macromolecular Substances
  • Humans
  • Gene Library