Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.
Publication
, Journal Article
Garrett, KM; Duman, RS; Saito, N; Blume, AJ; Vitek, MP; Tallman, JF
Published in: Biochem Biophys Res Commun
October 31, 1988
A cDNA clone of an alpha subunit of the human GABA-A receptor has been isolated. The human clone (pCLL800) contains 1055 nucleotides in an open reading frame and 260 nucleotides in the 5' non-coding region. The 351 amino acid sequence of this human alpha subunit shows 97% homology with its bovine counterpart. Hybridization of pCLL800 to Northern blots shows a 3.9/4.3 Kb RNA doublet in human cortex, rat whole brain, cortex, hippocampus, midbrain, olfactory bulb and cerebellum. Developmental studies show that the levels of the rat alpha mRNA increase between one and three weeks of age in a manner similar to the development of the benzodiazepine binding sites.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
October 31, 1988
Volume
156
Issue
2
Start / End Page
1039 / 1045
Location
United States
Related Subject Headings
- Tissue Distribution
- Sequence Homology, Nucleic Acid
- Receptors, GABA-A
- Rats
- RNA, Messenger
- Nucleic Acid Hybridization
- Molecular Sequence Data
- Humans
- DNA Probes
- DNA
Citation
APA
Chicago
ICMJE
MLA
NLM
Garrett, K. M., Duman, R. S., Saito, N., Blume, A. J., Vitek, M. P., & Tallman, J. F. (1988). Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor. Biochem Biophys Res Commun, 156(2), 1039–1045. https://doi.org/10.1016/s0006-291x(88)80949-5
Garrett, K. M., R. S. Duman, N. Saito, A. J. Blume, M. P. Vitek, and J. F. Tallman. “Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.” Biochem Biophys Res Commun 156, no. 2 (October 31, 1988): 1039–45. https://doi.org/10.1016/s0006-291x(88)80949-5.
Garrett KM, Duman RS, Saito N, Blume AJ, Vitek MP, Tallman JF. Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor. Biochem Biophys Res Commun. 1988 Oct 31;156(2):1039–45.
Garrett, K. M., et al. “Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.” Biochem Biophys Res Commun, vol. 156, no. 2, Oct. 1988, pp. 1039–45. Pubmed, doi:10.1016/s0006-291x(88)80949-5.
Garrett KM, Duman RS, Saito N, Blume AJ, Vitek MP, Tallman JF. Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor. Biochem Biophys Res Commun. 1988 Oct 31;156(2):1039–1045.
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
October 31, 1988
Volume
156
Issue
2
Start / End Page
1039 / 1045
Location
United States
Related Subject Headings
- Tissue Distribution
- Sequence Homology, Nucleic Acid
- Receptors, GABA-A
- Rats
- RNA, Messenger
- Nucleic Acid Hybridization
- Molecular Sequence Data
- Humans
- DNA Probes
- DNA