Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.

Publication ,  Journal Article
Garrett, KM; Duman, RS; Saito, N; Blume, AJ; Vitek, MP; Tallman, JF
Published in: Biochem Biophys Res Commun
October 31, 1988

A cDNA clone of an alpha subunit of the human GABA-A receptor has been isolated. The human clone (pCLL800) contains 1055 nucleotides in an open reading frame and 260 nucleotides in the 5' non-coding region. The 351 amino acid sequence of this human alpha subunit shows 97% homology with its bovine counterpart. Hybridization of pCLL800 to Northern blots shows a 3.9/4.3 Kb RNA doublet in human cortex, rat whole brain, cortex, hippocampus, midbrain, olfactory bulb and cerebellum. Developmental studies show that the levels of the rat alpha mRNA increase between one and three weeks of age in a manner similar to the development of the benzodiazepine binding sites.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 31, 1988

Volume

156

Issue

2

Start / End Page

1039 / 1045

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Sequence Homology, Nucleic Acid
  • Receptors, GABA-A
  • Rats
  • RNA, Messenger
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Humans
  • DNA Probes
  • DNA
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Garrett, K. M., Duman, R. S., Saito, N., Blume, A. J., Vitek, M. P., & Tallman, J. F. (1988). Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor. Biochem Biophys Res Commun, 156(2), 1039–1045. https://doi.org/10.1016/s0006-291x(88)80949-5
Garrett, K. M., R. S. Duman, N. Saito, A. J. Blume, M. P. Vitek, and J. F. Tallman. “Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.Biochem Biophys Res Commun 156, no. 2 (October 31, 1988): 1039–45. https://doi.org/10.1016/s0006-291x(88)80949-5.
Garrett KM, Duman RS, Saito N, Blume AJ, Vitek MP, Tallman JF. Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor. Biochem Biophys Res Commun. 1988 Oct 31;156(2):1039–45.
Garrett, K. M., et al. “Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.Biochem Biophys Res Commun, vol. 156, no. 2, Oct. 1988, pp. 1039–45. Pubmed, doi:10.1016/s0006-291x(88)80949-5.
Garrett KM, Duman RS, Saito N, Blume AJ, Vitek MP, Tallman JF. Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor. Biochem Biophys Res Commun. 1988 Oct 31;156(2):1039–1045.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 31, 1988

Volume

156

Issue

2

Start / End Page

1039 / 1045

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Sequence Homology, Nucleic Acid
  • Receptors, GABA-A
  • Rats
  • RNA, Messenger
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Humans
  • DNA Probes
  • DNA