Skip to main content

Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells.

Publication ,  Journal Article
Cayirlioglu, P; Ward, WO; Silver Key, SC; Duronio, RJ
Published in: Molecular and cellular biology
March 2003

Individual members of the E2F/DP protein family control cell cycle progression by acting predominantly as an activator or repressor of transcription. In Drosophila melanogaster the E2f1, E2f2, Dp, and Rbf1 genes all contribute to replication control in ovarian follicle cells, which become 16C polyploid and subsequently undergo chorion gene amplification late in oogenesis. Mutation of E2f2, Dp, or Rbf1 causes ectopic DNA replication throughout the follicle cell genome during gene amplification cycles. Here we show by both reverse transcription-PCR and DNA microarray analysis that the transcripts of prereplication complex (pre-RC) genes are elevated compared to the wild type in E2f2, Dp, and Rbf1 mutant follicle cells. For some genes the magnitude of this transcriptional derepression is greater in Rbf1 than in E2f2 mutants. These differences correlate with differences in the magnitude of the replication defects in follicle cells, which attain an inappropriate 32C DNA content in both Rbf1 and Dp mutants but not in E2f2 mutants. The ectopic genomic replication of E2f2 mutant follicle cells can be suppressed by reducing the Orc2, Orc5, or Mcm2 gene dose by half, indicating that small changes in pre-RC gene expression can affect DNA synthesis in these cells. We conclude that RBF1 forms complexes with both E2F1/DP and E2F2/DP that cooperate to repress the expression of pre-RC genes, which helps confine DNA synthesis to sites of gene amplification. In contrast, E2F1 and E2F2 repressors function redundantly for some genes in the embryo. Thus, the relative functional contributions of E2F1 and E2F2 to gene expression and cell cycle control depends on the developmental context.

Duke Scholars

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

March 2003

Volume

23

Issue

6

Start / End Page

2123 / 2134

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinoblastoma Protein
  • Phenotype
  • Ovarian Follicle
  • Oogenesis
  • Oligonucleotide Array Sequence Analysis
  • Mutation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cayirlioglu, P., Ward, W. O., Silver Key, S. C., & Duronio, R. J. (2003). Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. Molecular and Cellular Biology, 23(6), 2123–2134. https://doi.org/10.1128/mcb.23.6.2123-2134.2003
Cayirlioglu, Pelin, William O. Ward, S Catherine Silver Key, and Robert J. Duronio. “Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells.Molecular and Cellular Biology 23, no. 6 (March 2003): 2123–34. https://doi.org/10.1128/mcb.23.6.2123-2134.2003.
Cayirlioglu P, Ward WO, Silver Key SC, Duronio RJ. Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. Molecular and cellular biology. 2003 Mar;23(6):2123–34.
Cayirlioglu, Pelin, et al. “Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells.Molecular and Cellular Biology, vol. 23, no. 6, Mar. 2003, pp. 2123–34. Epmc, doi:10.1128/mcb.23.6.2123-2134.2003.
Cayirlioglu P, Ward WO, Silver Key SC, Duronio RJ. Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. Molecular and cellular biology. 2003 Mar;23(6):2123–2134.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

March 2003

Volume

23

Issue

6

Start / End Page

2123 / 2134

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinoblastoma Protein
  • Phenotype
  • Ovarian Follicle
  • Oogenesis
  • Oligonucleotide Array Sequence Analysis
  • Mutation