Skip to main content
Journal cover image

HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest.

Publication ,  Journal Article
Chowdhury, IH; Wang, X-F; Landau, NR; Robb, ML; Polonis, VR; Birx, DL; Kim, JH
Published in: Virology
January 20, 2003

The Vpr gene of human immunodeficiency virus type 1 (HIV-1) encodes a 14-kDa protein that prevents cell proliferation by causing arrest in the G2/M phase of the cell cycle. Here we report the first evidence that Vpr activates the expression and transcription of the cyclin-dependent kinase inhibitor p21/Waf1/Cip1 (hereafter p21), an inhibitor of the G1 and G2/M phase transitions in T lymphoid and myeloid cells. Vpr activated p21 protein expression in a dose-dependent manner. Vpr also caused a three- to eightfold induction of the p21 promoter. This induction was dose- and time-dependent and was comparable to levels of p21 induction induced by p53. Of note, Vpr activated p21 transcription in endogenous p53 positive cells, but not in p53-deleted or p53 nonfunctional cells. Vpr and p53 had an additive effect on p21 transcription. Mutational analysis indicated that wt Vpr, but not cell cycle inactive Vpr mutants, activated the p21 promoter. These data demonstrate that HIV-1 Vpr utilizes the cyclin-dependent kinase inhibitor p21, in addition to cdc2, to arrest cells in G2/M.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

January 20, 2003

Volume

305

Issue

2

Start / End Page

371 / 377

Location

United States

Related Subject Headings

  • Virology
  • Tumor Suppressor Protein p53
  • Transcriptional Activation
  • Mitosis
  • Humans
  • Gene Products, vpr
  • G2 Phase
  • Cyclins
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cell Line
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chowdhury, I. H., Wang, X.-F., Landau, N. R., Robb, M. L., Polonis, V. R., Birx, D. L., & Kim, J. H. (2003). HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest. Virology, 305(2), 371–377. https://doi.org/10.1006/viro.2002.1777
Chowdhury, Iqbal H., Xiao-Fan Wang, Nathaniel R. Landau, Merlin L. Robb, Victoria R. Polonis, Deborah L. Birx, and Jerome H. Kim. “HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest.Virology 305, no. 2 (January 20, 2003): 371–77. https://doi.org/10.1006/viro.2002.1777.
Chowdhury IH, Wang X-F, Landau NR, Robb ML, Polonis VR, Birx DL, et al. HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest. Virology. 2003 Jan 20;305(2):371–7.
Chowdhury, Iqbal H., et al. “HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest.Virology, vol. 305, no. 2, Jan. 2003, pp. 371–77. Pubmed, doi:10.1006/viro.2002.1777.
Chowdhury IH, Wang X-F, Landau NR, Robb ML, Polonis VR, Birx DL, Kim JH. HIV-1 Vpr activates cell cycle inhibitor p21/Waf1/Cip1: a potential mechanism of G2/M cell cycle arrest. Virology. 2003 Jan 20;305(2):371–377.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

January 20, 2003

Volume

305

Issue

2

Start / End Page

371 / 377

Location

United States

Related Subject Headings

  • Virology
  • Tumor Suppressor Protein p53
  • Transcriptional Activation
  • Mitosis
  • Humans
  • Gene Products, vpr
  • G2 Phase
  • Cyclins
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cell Line