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Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth.

Publication ,  Journal Article
Yingling, JM; Das, P; Savage, C; Zhang, M; Padgett, RW; Wang, XF
Published in: Proc Natl Acad Sci U S A
August 20, 1996

The dwarfin protein family has been genetically implicated in transforming growth factor beta (TGF-beta)-like signaling pathways in Drosophila and Caenorhabditis elegans. To investigate the role of these proteins in mammalian signaling pathways, we have isolated and studied two murine dwarfins, dwarfin-A and dwarfin-C. Using antibodies against dwarfin-A and dwarfin-C, we show that these two dwarfins and an immunogenically related protein, presumably also a dwarfin, are phosphorylated in a time- and dose-dependent manner in response to TGF-beta. Bone morphogenetic protein 2, a TGF-beta superfamily ligand, induces phosphorylation of only the related dwarfin protein. Thus, TGF-beta superfamily members may use overlapping yet distinct dwarfins to mediate their intracellular signals. Furthermore, transient overexpression of either dwarfin-A or dwarfin-C causes growth arrest, implicating the dwarfins in growth regulation. This work provides strong biochemical and preliminary functional evidence that dwarfin-A and dwarfin-C represent prototypic members of a family of mammalian proteins that may serve as mediators of signaling pathways for TGF-beta superfamily members.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

August 20, 1996

Volume

93

Issue

17

Start / End Page

8940 / 8944

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Trans-Activators
  • Smad5 Protein
  • Smad Proteins
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • Rats
  • Phosphorylation
  • Phosphoproteins
 

Citation

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Yingling, J. M., Das, P., Savage, C., Zhang, M., Padgett, R. W., & Wang, X. F. (1996). Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc Natl Acad Sci U S A, 93(17), 8940–8944. https://doi.org/10.1073/pnas.93.17.8940
Yingling, J. M., P. Das, C. Savage, M. Zhang, R. W. Padgett, and X. F. Wang. “Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth.Proc Natl Acad Sci U S A 93, no. 17 (August 20, 1996): 8940–44. https://doi.org/10.1073/pnas.93.17.8940.
Yingling JM, Das P, Savage C, Zhang M, Padgett RW, Wang XF. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8940–4.
Yingling, J. M., et al. “Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth.Proc Natl Acad Sci U S A, vol. 93, no. 17, Aug. 1996, pp. 8940–44. Pubmed, doi:10.1073/pnas.93.17.8940.
Yingling JM, Das P, Savage C, Zhang M, Padgett RW, Wang XF. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8940–8944.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

August 20, 1996

Volume

93

Issue

17

Start / End Page

8940 / 8944

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Trans-Activators
  • Smad5 Protein
  • Smad Proteins
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • Rats
  • Phosphorylation
  • Phosphoproteins