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Two-photon absorption and transient photothermal imaging of pigments in tissues

Publication ,  Journal Article
Ye, T; Fu, D; Matthews, TE; Hong, L; Simon, JD; Warren, WS
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
April 21, 2008

As a main pigment in skin tissues, melanin plays an important role in photo-protecting skin from UV radiation. However, melanogenesis may be altered due to disease or environmental factors; for example, sun exposure may cause damage and mutation of melanocytes and induce melanoma. Imaging pigmentation changes may provide invaluable information to catch the malignant transformation in its early stage and in turn improve the prognosis of patients. We have demonstrated previously that transmission mode, two-photon, one- or two-color absorption microscopy could provide remarkable contrast in imaging melanin in skin. In this report we demonstrate significantly improved sensitivity, so that we are now able to image in epi-mode (or back reflection) in two-photon absorption. This improvement makes possible for us to characterize the different types of pigmentation on the skin in vivo at virtually any location. Another finding is that we can also image transient photothermal dynamics due to the light absorption of melanin. By carefully choosing excitation and probe wavelengths, we might be able to image melanin in different structures under different micro-environments in skin, which could provide useful photochemical and photophysical insights in understanding how pigments are involved in photoprotection and photodamage of cells.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

April 21, 2008

Volume

6842
 

Citation

APA
Chicago
ICMJE
MLA
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Ye, T., Fu, D., Matthews, T. E., Hong, L., Simon, J. D., & Warren, W. S. (2008). Two-photon absorption and transient photothermal imaging of pigments in tissues. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 6842. https://doi.org/10.1117/12.764248
Ye, T., D. Fu, T. E. Matthews, L. Hong, J. D. Simon, and W. S. Warren. “Two-photon absorption and transient photothermal imaging of pigments in tissues.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE 6842 (April 21, 2008). https://doi.org/10.1117/12.764248.
Ye T, Fu D, Matthews TE, Hong L, Simon JD, Warren WS. Two-photon absorption and transient photothermal imaging of pigments in tissues. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2008 Apr 21;6842.
Ye, T., et al. “Two-photon absorption and transient photothermal imaging of pigments in tissues.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6842, Apr. 2008. Scopus, doi:10.1117/12.764248.
Ye T, Fu D, Matthews TE, Hong L, Simon JD, Warren WS. Two-photon absorption and transient photothermal imaging of pigments in tissues. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2008 Apr 21;6842.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

April 21, 2008

Volume

6842