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SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development.

Publication ,  Journal Article
Tsai, CC; Kao, HY; Yao, TP; McKeown, M; Evans, RM
Published in: Mol Cell
August 1999

The Drosophila ecdysone receptor (EcR)/ultraspiracle (USP) heterodimer is a key regulator in molting and metamorphoric processes, activating and repressing transcription in a sequence-specific manner. Here, we report the isolation of an EcR-interacting protein, SMRTER, which is structurally divergent but functionally similar to the vertebrate nuclear corepressors SMRT and N-CoR. SMRTER mediates repression by interacting with Sin3A, a repressor known to form a complex with the histone deacetylase Rpd3/HDAC. Importantly, we identify an EcR mutant allele that fails to bind SMRTER and is characterized by developmental defects and lethality. Together, these results reveal a novel nuclear receptor cofactor that exhibits evolutionary conservation in the mechanism to achieve repression and demonstrate the essential role of repression in hormone signaling.

Duke Scholars

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Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

August 1999

Volume

4

Issue

2

Start / End Page

175 / 186

Location

United States

Related Subject Headings

  • Vertebrates
  • Transfection
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Repressor Proteins
  • Recombinant Proteins
  • Receptors, Steroid
  • Receptors, Cytoplasmic and Nuclear
  • Nuclear Receptor Co-Repressor 2
  • Nuclear Receptor Co-Repressor 1
 

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Tsai, C. C., Kao, H. Y., Yao, T. P., McKeown, M., & Evans, R. M. (1999). SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development. Mol Cell, 4(2), 175–186. https://doi.org/10.1016/s1097-2765(00)80365-2
Tsai, C. C., H. Y. Kao, T. P. Yao, M. McKeown, and R. M. Evans. “SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development.Mol Cell 4, no. 2 (August 1999): 175–86. https://doi.org/10.1016/s1097-2765(00)80365-2.
Tsai CC, Kao HY, Yao TP, McKeown M, Evans RM. SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development. Mol Cell. 1999 Aug;4(2):175–86.
Tsai, C. C., et al. “SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development.Mol Cell, vol. 4, no. 2, Aug. 1999, pp. 175–86. Pubmed, doi:10.1016/s1097-2765(00)80365-2.
Tsai CC, Kao HY, Yao TP, McKeown M, Evans RM. SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development. Mol Cell. 1999 Aug;4(2):175–186.
Journal cover image

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

August 1999

Volume

4

Issue

2

Start / End Page

175 / 186

Location

United States

Related Subject Headings

  • Vertebrates
  • Transfection
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Repressor Proteins
  • Recombinant Proteins
  • Receptors, Steroid
  • Receptors, Cytoplasmic and Nuclear
  • Nuclear Receptor Co-Repressor 2
  • Nuclear Receptor Co-Repressor 1