Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.
Publication
, Journal Article
Balagaddé, FK; You, L; Hansen, CL; Arnold, FH; Quake, SR
Published in: Science (New York, N.Y.)
July 2005
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours.
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Published In
Science (New York, N.Y.)
DOI
EISSN
1095-9203
ISSN
0036-8075
Publication Date
July 2005
Volume
309
Issue
5731
Start / End Page
137 / 140
Related Subject Headings
- Time Factors
- Mutation
- Models, Biological
- Microfluidics
- Mathematics
- Isopropyl Thiogalactoside
- Genes, Bacterial
- General Science & Technology
- Feedback, Physiological
- Escherichia coli
Citation
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ICMJE
MLA
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Balagaddé, F. K., You, L., Hansen, C. L., Arnold, F. H., & Quake, S. R. (2005). Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Science (New York, N.Y.), 309(5731), 137–140. https://doi.org/10.1126/science.1109173
Balagaddé, Frederick K., Lingchong You, Carl L. Hansen, Frances H. Arnold, and Stephen R. Quake. “Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.” Science (New York, N.Y.) 309, no. 5731 (July 2005): 137–40. https://doi.org/10.1126/science.1109173.
Balagaddé FK, You L, Hansen CL, Arnold FH, Quake SR. Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Science (New York, NY). 2005 Jul;309(5731):137–40.
Balagaddé, Frederick K., et al. “Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.” Science (New York, N.Y.), vol. 309, no. 5731, July 2005, pp. 137–40. Epmc, doi:10.1126/science.1109173.
Balagaddé FK, You L, Hansen CL, Arnold FH, Quake SR. Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. Science (New York, NY). 2005 Jul;309(5731):137–140.
Published In
Science (New York, N.Y.)
DOI
EISSN
1095-9203
ISSN
0036-8075
Publication Date
July 2005
Volume
309
Issue
5731
Start / End Page
137 / 140
Related Subject Headings
- Time Factors
- Mutation
- Models, Biological
- Microfluidics
- Mathematics
- Isopropyl Thiogalactoside
- Genes, Bacterial
- General Science & Technology
- Feedback, Physiological
- Escherichia coli