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Ontogeny and localization of TGF-beta type I receptor expression during lung development.

Publication ,  Journal Article
Zhao, Y; Young, SL; McIntosh, JC; Steele, MP; Silbajoris, R
Published in: Am J Physiol Lung Cell Mol Physiol
June 2000

Transforming growth factor (TGF)-beta is a family of multifunctional cytokines controlling cell growth, differentiation, and extracellular matrix deposition in the lung. The biological effects of TGF-beta are mediated by type I (TbetaR-I) and II (TbetaR-II) receptors. Our previous studies show that the expression of TbetaR-II is highly regulated in a spatial and temporal fashion during lung development. In the present studies, we investigated the temporal-spatial pattern and cellular expression of TbetaR-I during lung development. The expression level of TbetaR-I mRNA in rat lung at different embryonic and postnatal stages was analyzed by Northern blotting. TbetaR-I mRNA was expressed in fetal rat lungs in early development and then decreased as development proceeded. The localization of TbetaR-I in fetal and postnatal rat lung tissues was investigated by using in situ hybridization performed with an antisense RNA probe. TbetaR-I mRNA was present in the mesenchyme and epithelium of gestational day 14 rat lungs. An intense TbetaR-I signal was observed in the epithelial lining of the developing bronchi. In gestational day 16 lungs, the expression of TbetaR-I mRNA was increased in the mesenchymal tissue. The epithelium in both the distal and proximal bronchioles showed a similar level of TbetaR-I expression. In postnatal lungs, TbetaR-I mRNA was detected in parenchymal tissues and blood vessels. We further studied the expression of TbetaR-I in cultured rat lung cells. TbetaR-I was expressed by cultured rat lung fibroblasts, microvascular endothelial cells, and alveolar epithelial cells. These studies demonstrate a differential regulation and localization of TbetaR-I that is different from that of TbetaR-II during lung development. TbetaR-I, TbetaR-II, and TGF-beta isoforms exhibit distinct but overlapping patterns of expression during lung development. This implies a distinct role for TbetaR-I in mediating TGF-beta signal transduction during lung development.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

June 2000

Volume

278

Issue

6

Start / End Page

L1231 / L1239

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Respiratory System
  • Receptors, Transforming Growth Factor beta
  • Receptor, Transforming Growth Factor-beta Type I
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Serine-Threonine Kinases
  • Lung
  • In Situ Hybridization
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhao, Y., Young, S. L., McIntosh, J. C., Steele, M. P., & Silbajoris, R. (2000). Ontogeny and localization of TGF-beta type I receptor expression during lung development. Am J Physiol Lung Cell Mol Physiol, 278(6), L1231–L1239. https://doi.org/10.1152/ajplung.2000.278.6.L1231
Zhao, Y., S. L. Young, J. C. McIntosh, M. P. Steele, and R. Silbajoris. “Ontogeny and localization of TGF-beta type I receptor expression during lung development.Am J Physiol Lung Cell Mol Physiol 278, no. 6 (June 2000): L1231–39. https://doi.org/10.1152/ajplung.2000.278.6.L1231.
Zhao Y, Young SL, McIntosh JC, Steele MP, Silbajoris R. Ontogeny and localization of TGF-beta type I receptor expression during lung development. Am J Physiol Lung Cell Mol Physiol. 2000 Jun;278(6):L1231–9.
Zhao, Y., et al. “Ontogeny and localization of TGF-beta type I receptor expression during lung development.Am J Physiol Lung Cell Mol Physiol, vol. 278, no. 6, June 2000, pp. L1231–39. Pubmed, doi:10.1152/ajplung.2000.278.6.L1231.
Zhao Y, Young SL, McIntosh JC, Steele MP, Silbajoris R. Ontogeny and localization of TGF-beta type I receptor expression during lung development. Am J Physiol Lung Cell Mol Physiol. 2000 Jun;278(6):L1231–L1239.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

June 2000

Volume

278

Issue

6

Start / End Page

L1231 / L1239

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Respiratory System
  • Receptors, Transforming Growth Factor beta
  • Receptor, Transforming Growth Factor-beta Type I
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Serine-Threonine Kinases
  • Lung
  • In Situ Hybridization