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Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy.

Publication ,  Journal Article
Kulis, M; Macqueen, I; Li, Y; Guo, R; Zhong, X-P; Burks, AW
Published in: J Allergy Clin Immunol
September 2012

BACKGROUND: IgE-mediated allergic reactions to cashews and other nuts can trigger life-threatening anaphylaxis. Proactive therapies to decrease reaction severity do not exist. OBJECTIVES: We aimed to determine the efficacy of pepsin-digested cashew proteins used as immunotherapy in a murine model of cashew allergy. METHODS: Mice were sensitized to cashew and then underwent challenges with digested or native cashew allergens to assess the allergenicity of the protein preparations. Using native or pepsinized cashew proteins, mice underwent oral or intraperitoneal sensitization protocols to determine the immunogenic properties of the protein preparations. Finally, cashew-sensitized mice underwent an immunotherapy protocol with native or pepsinized cashew proteins and subsequent provocation challenges. RESULTS: Pepsinized cashew proteins elicited weaker allergic reactions than native cashew proteins but importantly retained the ability to stimulate cellular proliferation and cytokine production. Mice sensitized with pepsinized proteins reacted on challenge with native allergens, demonstrating that pepsinized allergens retain immunogenicity in vivo. Immunotherapy with pepsinized cashew allergens significantly decreased allergic symptoms and body temperature decrease relative to placebo after challenge with native and pepsinized proteins. Immunologic changes were comparable after immunotherapy with native or pepsinized allergens: T(H)2-type cytokine secretion from splenocytes was decreased, whereas specific IgG(1) and IgG(2a) levels were increased. CONCLUSIONS: Pepsinized cashew proteins are effective in treating cashew allergy in mice and appear to work through the same mechanisms as native protein immunotherapy.

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Published In

J Allergy Clin Immunol

DOI

EISSN

1097-6825

Publication Date

September 2012

Volume

130

Issue

3

Start / End Page

716 / 723

Location

United States

Related Subject Headings

  • Th2 Cells
  • Plant Proteins
  • Peptide Fragments
  • Pepsin A
  • Nut Hypersensitivity
  • Mice, Inbred C3H
  • Mice
  • Immunoglobulin G
  • Female
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kulis, M., Macqueen, I., Li, Y., Guo, R., Zhong, X.-P., & Burks, A. W. (2012). Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy. J Allergy Clin Immunol, 130(3), 716–723. https://doi.org/10.1016/j.jaci.2012.05.044
Kulis, Mike, Ian Macqueen, Yifan Li, Rishu Guo, Xiao-Ping Zhong, and A Wesley Burks. “Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy.J Allergy Clin Immunol 130, no. 3 (September 2012): 716–23. https://doi.org/10.1016/j.jaci.2012.05.044.
Kulis M, Macqueen I, Li Y, Guo R, Zhong X-P, Burks AW. Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy. J Allergy Clin Immunol. 2012 Sep;130(3):716–23.
Kulis, Mike, et al. “Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy.J Allergy Clin Immunol, vol. 130, no. 3, Sept. 2012, pp. 716–23. Pubmed, doi:10.1016/j.jaci.2012.05.044.
Kulis M, Macqueen I, Li Y, Guo R, Zhong X-P, Burks AW. Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy. J Allergy Clin Immunol. 2012 Sep;130(3):716–723.
Journal cover image

Published In

J Allergy Clin Immunol

DOI

EISSN

1097-6825

Publication Date

September 2012

Volume

130

Issue

3

Start / End Page

716 / 723

Location

United States

Related Subject Headings

  • Th2 Cells
  • Plant Proteins
  • Peptide Fragments
  • Pepsin A
  • Nut Hypersensitivity
  • Mice, Inbred C3H
  • Mice
  • Immunoglobulin G
  • Female
  • Disease Models, Animal