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Regulation of T cell receptor-induced activation of the Ras-ERK pathway by diacylglycerol kinase zeta.

Publication ,  Journal Article
Zhong, X-P; Hainey, EA; Olenchock, BA; Zhao, H; Topham, MK; Koretzky, GA
Published in: J Biol Chem
August 23, 2002

T cell development in the thymus and activation of mature T cells in the periphery depend on signals stimulated by engagement of the T cell antigen receptor (TCR). Among the second messenger cascades initiated by TCR ligation include the phosphatidylinositol pathway where the membrane phospholipid, phosphatidylinositol 4,5-bisphosphate, is hydrolyzed to inositol 1,4,5-trisphosphate and diacylglycerol (DAG). Inositol 1,4,5-trisphosphate signals a rise in intracellular free calcium, leading to translocation of nuclear factor of activated T cells into the nucleus. DAG activates RasGRP and protein kinase C theta. Because both RasGRP and protein kinase C theta are essential for thymocyte and T cell function, it is critical to understand how DAG is regulated. In this report, we demonstrate expression of DAG kinase zeta (DGKzeta, the enzyme that catalyzes the conversion of DAG to phosphatidic acid) in multiple lymphoid organs, with highest expression observed within the T cell compartment. Overexpression studies in Jurkat T cells indicate that DGKzeta interferes with TCR-induced Ras and ERK activation, AP-1 induction, and expression of the activation marker CD69. In contrast, TCR-stimulated calcium influx is not altered. Mutational analysis indicates that the kinase and DAG binding domains, but not the ankyrin repeats of DGKzeta, are required for its inhibitory effects. Collectively these studies demonstrate a potential role of DGKzeta to function as a selective negative regulator of DAG signaling on T cell activation and provide the first structure/function analysis of this enzyme in T cells.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

August 23, 2002

Volume

277

Issue

34

Start / End Page

31089 / 31098

Location

United States

Related Subject Headings

  • ras Proteins
  • Transcription Factor AP-1
  • Tetradecanoylphorbol Acetate
  • Receptors, Antigen, T-Cell
  • Mitogen-Activated Protein Kinases
  • Lectins, C-Type
  • Jurkat Cells
  • Isoenzymes
  • Humans
  • Diacylglycerol Kinase
 

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Zhong, X.-P., Hainey, E. A., Olenchock, B. A., Zhao, H., Topham, M. K., & Koretzky, G. A. (2002). Regulation of T cell receptor-induced activation of the Ras-ERK pathway by diacylglycerol kinase zeta. J Biol Chem, 277(34), 31089–31098. https://doi.org/10.1074/jbc.M203818200
Zhong, Xiao-Ping, Ehmonie A. Hainey, Benjamin A. Olenchock, Haoran Zhao, Matthew K. Topham, and Gary A. Koretzky. “Regulation of T cell receptor-induced activation of the Ras-ERK pathway by diacylglycerol kinase zeta.J Biol Chem 277, no. 34 (August 23, 2002): 31089–98. https://doi.org/10.1074/jbc.M203818200.
Zhong X-P, Hainey EA, Olenchock BA, Zhao H, Topham MK, Koretzky GA. Regulation of T cell receptor-induced activation of the Ras-ERK pathway by diacylglycerol kinase zeta. J Biol Chem. 2002 Aug 23;277(34):31089–98.
Zhong, Xiao-Ping, et al. “Regulation of T cell receptor-induced activation of the Ras-ERK pathway by diacylglycerol kinase zeta.J Biol Chem, vol. 277, no. 34, Aug. 2002, pp. 31089–98. Pubmed, doi:10.1074/jbc.M203818200.
Zhong X-P, Hainey EA, Olenchock BA, Zhao H, Topham MK, Koretzky GA. Regulation of T cell receptor-induced activation of the Ras-ERK pathway by diacylglycerol kinase zeta. J Biol Chem. 2002 Aug 23;277(34):31089–31098.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

August 23, 2002

Volume

277

Issue

34

Start / End Page

31089 / 31098

Location

United States

Related Subject Headings

  • ras Proteins
  • Transcription Factor AP-1
  • Tetradecanoylphorbol Acetate
  • Receptors, Antigen, T-Cell
  • Mitogen-Activated Protein Kinases
  • Lectins, C-Type
  • Jurkat Cells
  • Isoenzymes
  • Humans
  • Diacylglycerol Kinase