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Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta.

Publication ,  Journal Article
Bomar, MG; Pai, M-T; Tzeng, S-R; Li, SS-C; Zhou, P
Published in: EMBO Rep
March 2007

The ubiquitin-binding zinc finger (UBZ) domain of human DNA Y-family polymerase (pol) eta is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. Here, we report the solution structure of the pol eta UBZ domain and its interaction with ubiquitin. We show that the UBZ domain adopts a classical C(2)H(2) zinc-finger structure characterized by a betabetaalpha fold. Nuclear magnetic resonance titration maps the binding interfaces between UBZ and ubiquitin to the alpha-helix of the UBZ domain and the canonical hydrophobic surface of ubiquitin defined by residues L8, I44 and V70. Although the UBZ domain binds ubiquitin through a single alpha-helix, in a manner similar to the inverted ubiquitin-interacting motif, its structure is distinct from previously characterized ubiquitin-binding domains. The pol eta UBZ domain represents a novel member of the C(2)H(2) zinc finger family that interacts with ubiquitin to regulate translesion synthesis.

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Published In

EMBO Rep

DOI

ISSN

1469-221X

Publication Date

March 2007

Volume

8

Issue

3

Start / End Page

247 / 251

Location

England

Related Subject Headings

  • Zinc Fingers
  • Ubiquitin
  • Spin Labels
  • Sequence Alignment
  • Molecular Sequence Data
  • Models, Molecular
  • Mass Spectrometry
  • Magnetic Resonance Spectroscopy
  • Humans
  • Developmental Biology
 

Citation

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ICMJE
MLA
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Bomar, M. G., Pai, M.-T., Tzeng, S.-R., Li, S.-C., & Zhou, P. (2007). Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta. EMBO Rep, 8(3), 247–251. https://doi.org/10.1038/sj.embor.7400901
Bomar, Martha G., Ming-Tao Pai, Shiou-Ru Tzeng, Shawn Shun-Cheng Li, and Pei Zhou. “Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta.EMBO Rep 8, no. 3 (March 2007): 247–51. https://doi.org/10.1038/sj.embor.7400901.
Bomar MG, Pai M-T, Tzeng S-R, Li SS-C, Zhou P. Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta. EMBO Rep. 2007 Mar;8(3):247–51.
Bomar, Martha G., et al. “Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta.EMBO Rep, vol. 8, no. 3, Mar. 2007, pp. 247–51. Pubmed, doi:10.1038/sj.embor.7400901.
Bomar MG, Pai M-T, Tzeng S-R, Li SS-C, Zhou P. Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta. EMBO Rep. 2007 Mar;8(3):247–251.
Journal cover image

Published In

EMBO Rep

DOI

ISSN

1469-221X

Publication Date

March 2007

Volume

8

Issue

3

Start / End Page

247 / 251

Location

England

Related Subject Headings

  • Zinc Fingers
  • Ubiquitin
  • Spin Labels
  • Sequence Alignment
  • Molecular Sequence Data
  • Models, Molecular
  • Mass Spectrometry
  • Magnetic Resonance Spectroscopy
  • Humans
  • Developmental Biology