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Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies.

Publication ,  Journal Article
Papadaki, M; Bursac, N; Langer, R; Merok, J; Vunjak-Novakovic, G; Freed, LE
Published in: American journal of physiology. Heart and circulatory physiology
January 2001

The primary aim of this study was to relate molecular and structural properties of in vitro reconstructed cardiac muscle with its electrophysiological function using an in vitro model system based on neonatal rat cardiac myocytes, three-dimensional polymeric scaffolds, and bioreactors. After 1 wk of cultivation, we found that engineered cardiac muscle contained a 120- to 160-microm-thick peripheral region with cardiac myocytes that were electrically connected through gap junctions and sustained macroscopically continuous impulse propagation over a distance of 5 mm. Molecular, structural, and electrophysiological properties were found to be interrelated and depended on specific model system parameters such as the tissue culture substrate, bioreactor, and culture medium. Native tissue and the best experimental group (engineered cardiac muscle cultivated using laminin-coated scaffolds, rotating bioreactors, and low-serum medium) were comparable with respect to the conduction velocity of propagated electrical impulses and spatial distribution of connexin43. Furthermore, the structural and electrophysiological properties of the engineered cardiac muscle, such as cellularity, conduction velocity, maximum signal amplitude, capture rate, and excitation threshold, were significantly improved compared with our previous studies.

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Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

January 2001

Volume

280

Issue

1

Start / End Page

H168 / H178

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Myosin Heavy Chains
  • Myocardium
  • Microscopy, Electron
  • Microscopy, Confocal
  • Laminin
  • L-Lactate Dehydrogenase
  • Isoenzymes
  • Heart
 

Citation

APA
Chicago
ICMJE
MLA
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Papadaki, M., Bursac, N., Langer, R., Merok, J., Vunjak-Novakovic, G., & Freed, L. E. (2001). Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies. American Journal of Physiology. Heart and Circulatory Physiology, 280(1), H168–H178. https://doi.org/10.1152/ajpheart.2001.280.1.h168
Papadaki, M., N. Bursac, R. Langer, J. Merok, G. Vunjak-Novakovic, and L. E. Freed. “Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies.American Journal of Physiology. Heart and Circulatory Physiology 280, no. 1 (January 2001): H168–78. https://doi.org/10.1152/ajpheart.2001.280.1.h168.
Papadaki M, Bursac N, Langer R, Merok J, Vunjak-Novakovic G, Freed LE. Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies. American journal of physiology Heart and circulatory physiology. 2001 Jan;280(1):H168–78.
Papadaki, M., et al. “Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies.American Journal of Physiology. Heart and Circulatory Physiology, vol. 280, no. 1, Jan. 2001, pp. H168–78. Epmc, doi:10.1152/ajpheart.2001.280.1.h168.
Papadaki M, Bursac N, Langer R, Merok J, Vunjak-Novakovic G, Freed LE. Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies. American journal of physiology Heart and circulatory physiology. 2001 Jan;280(1):H168–H178.

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

January 2001

Volume

280

Issue

1

Start / End Page

H168 / H178

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Myosin Heavy Chains
  • Myocardium
  • Microscopy, Electron
  • Microscopy, Confocal
  • Laminin
  • L-Lactate Dehydrogenase
  • Isoenzymes
  • Heart