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High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.

Publication ,  Journal Article
Wu, JY; Siegel, LM; Kredich, NM
Published in: J Bacteriol
January 1991

The flavoprotein and hemoprotein components of Escherichia coli B NADPH-sulfite reductase are encoded by cysJ and cysI, respectively. Plasmids containing these two genes overexpressed flavoprotein catalytic activity and apohemoprotein by 13- to 35-fold, but NADPH-sulfite reductase holoenzyme activity was increased only 3-fold. Maximum overexpression of holoenzyme activity was achieved by the inclusion in such plasmids of Salmonella typhimurium cysG, which encodes a uroporphyrinogen III methyltransferase required for the synthesis of siroheme, a cofactor for the hemoprotein. Thus, cofactor deficiency, in this case siroheme, can limit overexpression of a cloned enzyme. Catalytically active holoenzyme accounted for 10% of total soluble protein in a host containing cloned cysJ, cysI, and cysG. A 5.3-kb DNA fragment containing S. typhimurium cysG was sequenced, and the open reading frame corresponding to cysG was identified by subcloning and by identifying plasmid-encoded peptides in maxicells. Comparison with the sequence reported for the E. coli cysG region (J. A. Cole, unpublished data; GenBank sequence ECONIRBC) indicates a gene order of nirB-nirC-cysG in the cloned S. typhimurium fragment. In addition, two open reading frames of unknown identity were found immediately downstream of cysG. One of these contains 11 direct repeats of 33 nucleotides each, which correspond to the consensus amino acid sequence Asp-Asp-Val-Thr-Pro-Pro-Asp-Asp-Ser-Gly-Asp.

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Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1991

Volume

173

Issue

1

Start / End Page

325 / 333

Location

United States

Related Subject Headings

  • Sulfite Reductase (NADPH)
  • Salmonella typhimurium
  • Restriction Mapping
  • Plasmids
  • Oxidoreductases Acting on Sulfur Group Donors
  • Molecular Sequence Data
  • Microbiology
  • Methyltransferases
  • Heme
  • Gene Expression Regulation, Bacterial
 

Citation

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Wu, J. Y., Siegel, L. M., & Kredich, N. M. (1991). High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor. J Bacteriol, 173(1), 325–333. https://doi.org/10.1128/jb.173.1.325-333.1991
Wu, J. Y., L. M. Siegel, and N. M. Kredich. “High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.J Bacteriol 173, no. 1 (January 1991): 325–33. https://doi.org/10.1128/jb.173.1.325-333.1991.
Wu, J. Y., et al. “High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.J Bacteriol, vol. 173, no. 1, Jan. 1991, pp. 325–33. Pubmed, doi:10.1128/jb.173.1.325-333.1991.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1991

Volume

173

Issue

1

Start / End Page

325 / 333

Location

United States

Related Subject Headings

  • Sulfite Reductase (NADPH)
  • Salmonella typhimurium
  • Restriction Mapping
  • Plasmids
  • Oxidoreductases Acting on Sulfur Group Donors
  • Molecular Sequence Data
  • Microbiology
  • Methyltransferases
  • Heme
  • Gene Expression Regulation, Bacterial